| 甘薯毛状根植株再生研究 |
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| 引用本文： | 孙敏,陈敏,廖志华,王和勇.甘薯毛状根植株再生研究[J].西南师范大学学报(自然科学版),2000,25(5):543-546. |
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| 作者姓名： | 孙敏 陈敏 廖志华 王和勇 |
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| 作者单位： | 西南师范大学生命科学学院,重庆 400715 |
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| 摘 要： | 用发根农杆菌Ｒ１０００菌株感梁甘薯（渝薯３４品种），获得转化毛状根，从毛状根中选择出了５个无性系，当毛状根培养在含有２ｍｇ／Ｌ６－ＢＡ＋１ｍｏｌ／Ｌ玉米素的ＭＳ培养基上诱导出了愈伤组织，当愈伤组织培养在含有１ｍｇ／ＬＫＴ＋１ｍｇ／Ｌ６－ＢＡ＋０．５ｍｇ／ＬＩＡＡ的培养基上，３周以后获得了再生植株，用随机及增多态性ＤＮＡ（ＲＡＰＤ）分子标记检测出了再生植株的多态笥。
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| 关 键 词： | 甘薯 毛状根 植株再生 愈伤组织 |
| 文章编号： | 1000-5471(2000)05-0543-04 |
| 修稿时间： | 2000年5月15日 |
Plantlet regeneration from hairy roots of sweetpotato(Ipomoea batatas(L.)Lam.) |
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| SUN\ Min,CHEN\ Min,LIAO Zhi hua,WANG He yong.Plantlet regeneration from hairy roots of sweetpotato(Ipomoea batatas(L.)Lam.)[J].Journal of Southwest China Normal University(Natural Science),2000,25(5):543-546. |
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| Authors: | SUN\ Min CHEN\ Min LIAO Zhi hua WANG He yong |
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| Abstract: | Transformed hairy roots were induced in sweet potato ( Ipomoea batatas ) by Agrobacterium rhizogenes strain R1000.Five clones were selected from hairy roots.Callus was induced when hairy roots were cultured on the medium containing 2 mg/L 6 BA and l mg/L zeatin.Plantlets were regenerated from callus in 3 weeks on the media containing l mg/L KT,l mg/L 6 BA and 0 5mg/L IAA.The polymorphism of regenerated plantlets was detected by the use of a RAPD marker. |
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| Keywords: | sweet potato hairy root plantlet regeneration RAPD marker |
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