| 龙血竭HPLC指纹图谱研究 |
| |
| 引用本文： | 徐玉玲,梁慧,凌娅,杜思雯,伍利华,苟小军. 龙血竭HPLC指纹图谱研究[J]. 成都大学学报(自然科学版), 2014, 0(1): 1-3 |
| |
| 作者姓名： | 徐玉玲 梁慧 凌娅 杜思雯 伍利华 苟小军 |
| |
| 作者单位： | [1]成都大学实验技术中心,四川成都610106 [2]江苏康缘药业股份有限公司,江苏连云港222001 [3]广西中医药大学药学院,广西南宁530001 [4]成都大学生物产业学院,四川成都610106 |
| |
| 摘 要： | 为建立龙血竭指纹图谱质量控制方法,采用HPLC法测定,液相色谱仪Agilent 1100,色谱柱为Waters Xterra C184.6×250 mm,5μm;流动相为0.1%的磷酸溶液和乙腈梯度洗脱,流速为0.7 mL/min,检测波长为280 nm,理论板数按参照物(龙血素B)峰计算不低于6000.实验结果表明,10批成品指纹图谱与对照指纹图谱计算相似度,其结果均大于0.90.该方法稳定可行,可用于龙血竭质量控制.
|
| 关 键 词： | 龙血竭 HPLC 指纹图谱 相似度 质量控制 |
Study on HPLC Fingerprint of Dracaena Cochinchinensis |
| |
| XU Yuling,LIANG Hui,LING Ya,DU SiwenI,WU Lihuaz,GOU Xiaojun. Study on HPLC Fingerprint of Dracaena Cochinchinensis[J]. Journal of Chengdu University (Natural Science), 2014, 0(1): 1-3 |
| |
| Authors: | XU Yuling LIANG Hui LING Ya DU SiwenI WU Lihuaz GOU Xiaojun |
| |
| Affiliation: | 4 (1 .Experimental Technology Center, Chengdu University, Chengdu 610106, China; 2.Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China; 3. College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530001, China; 4. School of Biological Industry, Chengdu University, Chengdu 610106, China) |
| |
| Abstract: | To establish fingerprint quality control method of Dracaena cochinchinensis, this paper adopts HPLC method for determination. Agilent I100 liquid chromatography and the Waters Xterra Cls （4.6 mm × 250 mm, 5 tma）are used. The mobile phase is a linear gradient of acetonitrile and 0.1% Oahophosphoric acid at the flow rate of 0.7 mL· min- 1. UV detection wavelength is set at 280 nm. The number of theoreti- cal plates is not less than 6 000 according to reference compound （Loureirin B） peak. The experimental re- suits show that the overall similarity is greater than 0.9 by comparing the fingerprints of 10 batches of sam- pies and the representative f＇mgerprints. We conclude that this method is stable and feasible, which can be used for research and quality control of Dracaena cochinchinensis. |
| |
| Keywords: | Dracaena cochinchinensis HPLC fingerprint similarity quality control |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
