A common ancestor for a subunit in the mitochondrial proton-translocating NADH:ubiquinone oxidoreductase (complex I) and short-chain dehydrogenases/reductases |
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Authors: | M. E. Baker W. N. Grundy C. P. Elkan |
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Affiliation: | (1) Department of Medicine, 0823, University of California, San Diego, 9500 Gilman Drive, La Jolla (California 92093-0823, USA), Fax +1 619 534 1424, e-mail: mbaker@ucsd.edu, US;(2) Department of Computer Science, University of California, San Diego, 9500 Gilman Drive, La Jolla (California 92093-0623, USA), US |
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Abstract: | The proton-translocating NADH:ubiquinone oxidoreductase or complex I is located in the inner membranes of mitochondria, where it catalyzes the transfer of electrons from NADH to ubiquinone. Here we report that one of the subunits in complex I is homologous to short-chain dehydrogenases and reductases, a family of enzymes with diverse activities that include metabolizing steroids, prostaglandins and nucleotide sugars. We discovered that a subunit of complex I in human, cow, Neurospora crassa and Aquifex aeolius is homologous to nucleotide-sugar epimerases and hydroxysteroid dehydrogenases while seeking distant homologs of these enzymes with a hidden Markov model-based search of Genpept. This homology allows us to use information from the solved three-dimensional structures of nucleotide-sugar epimerases and hydroxysteroid dehydrogenases and our motif analysis of these enzymes to predict functional domains on their homologs in complex I. Received 26 November 1998; received after revision 12 January 1999; accepted 12 January 1999 |
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Keywords: | . NADH:ubiquinone oxidoreductase short-chain dehydrogenases/reductases nucleotide-sugar epimerases hydroxysteroid dehydrogenases evolution. |
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