敌枯双(Bis-A-TDA)对果蝇(Drosophila melanogaster)的胚胎毒性及烟酰胺的解毒作用

将野生型果蝇分别培养于含0,10,100,150,200,25O,35O,500,700,1000ppm敌枯双的果蝇培养基上,在25℃交配、产卵6d.子代成蝇羽化后,每天检查其外部畸形并计数.解毒试验组加烟酰胺(NA)、敌枯双(Bis-A-TDA)各200ppm于培养基中.实验结果表明:(1)敌枯双对果蝇的胚胎毒性随剂量增加而上升.(2)烟酰胺能部分解除敌枯双对果蝇的胚胎毒性.(3)低剂量敌枯双(10—100ppm)处理,能增加子代成蝇的羽化数,有促进作用.

EMBRYONIC TOXICITY OF DI-KU-SHUANG (BIS-A-TDA) ON DROSOPHILA MELANOGASTER AND THE DETOXICATION OF NIACIAMIDE

The known teratogen, Eis A TDA (N, N' Methylene-bis (2 amino 1, 3, 4 thiadiazole)), was tested for influence on embryonic development of D. meianogaster. Treatment consisted of rearing the fly larvae in media containing Pis A TDA concentrations of 0, 10, 100, 150, 200, 250, 350, 500, 750 and l000ppm. Emerging flies were counted and inspected for gross malformation. Toxicity inhibition test was conducted with medium containing inhibitor niacinamide and Bis A TDA both on concentra Men of 200prm. The results show: 1. there is a line relationship between the ombryonic toxieity of Eis A-TDA and its dosage. When the dosage rises from 150 to 350ppm, the number of om flies decreases and malformation flies increase dramatically. Among larvae, rua and adult fly, pupa is most sensitive to Bis A TDA.2. niacinamide can partly inhibit the embryonie toxicity of Bis A TDA in D. melanogaster.3. low concentration of Bis A TDA (10 to 100ppm) can enhance cmbryonic development of D. melanogaster.