Studies on reduced extent method for inhibited thermokinetics of enzyme-catalyzed reaction |
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| Authors: | Xiong Ya Wu Dingquan Qu Songsheng Du Yumin |
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| Affiliation: | (1) Department of Chemistry, Wuhan University, 430072 Wuhan, China;(2) Department of Environmental Science, Wuhan University, 430072 Wuhan, China |
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| Abstract: | The thermokinetic reduced extent equations of reversible inhibitions for Michaiels-Menten enzymatic reaction were deduced, and then the criteria for distinguishing inhibition type was given and the methods for calculating kinetic parameters,K M,K i andv m were suggested. This theory was applied to inverstigate the inhibited thermokinetics of laccase-catalyzed oxidation ofo-dihydroxybenzene bym-dihydroxybenzene. The experimental results show the inhibition belongs to reversible competitive type,K M=6.224×10−3 mol·L−1,K i=2.363×10−2 mol·L−1. Xiong Ya: born Sep. 1961, Ph. D. graduate student. Curent research interest is in biothermochemistry research Supported by the National Natural Science Foundation of China |
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| Keywords: | thermokinetics enzyme-catalyzed reaction inhibition reduced extent method laccase |
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