|
|||||
|
|
| 利用SSR及ISSR分子标记研究苜蓿属及其近缘植物的亲缘关系 | |
| 引用本文： | 李飞飞,羊海军,崔大方.利用SSR及ISSR分子标记研究苜蓿属及其近缘植物的亲缘关系[J].中山大学学报(自然科学版),2014,53(1). |
| 作者姓名： | 李飞飞 羊海军 崔大方 |
| 作者单位： | 1. 中央民族大学生命与环境科学学院，北京 100081； 2. 华南农业大学公共基础实验教学中心，广东 广州 510642； 3. 华南农业大学林学院，广东 广州 510642 |
| 基金项目： | 国家自然科学基金资助项目(30670145) |
| 摘 要： | 利用30对SSR引物和12个ISSR引物对苜蓿属(Medicago)及其近缘植物共11个物种的亲缘关系进行研究。通过遗传多样性研究及UPGMA聚类分析显示,"阔荚类群"(subgen.Platycapos)的4个种在两种分子标记下都聚为一支,符合其相似的形态学特征;"阔荚类群"在SSR分子标记中与胡卢巴属(Trigonella)聚成一支,网脉胡卢巴(T.cancellata)在ISSR分子标记中聚在"阔荚类群"中;两种分子标记中,"紫花苜蓿复合体"(Medicago sativa complex)3个种间的遗传距离都最短;南苜蓿(M.polymorpha)在两种分子标记下的聚类结果有所不同,SSR分子标记下与胡卢巴属的亲缘关系更近。研究说明苜蓿属与胡卢巴属具有很近的亲缘关系,苜蓿属内不同物种与胡卢巴属的亲缘关系有远有近,其中苜蓿属中的"阔荚类群"与胡卢巴属的关系最近,苜蓿属中其他物种与胡卢巴属的亲缘关系较远。 |
| 关 键 词： | 苜蓿属 近缘植物 分子标记 亲缘关系 |
| 收稿时间： | 2013-07-21; |
Genetic Relationships Among 11 Species of Medicago and Relative Species Using Microsatellite (SSR and ISSR) Markers |
|
| LI Feifei,YANG Haijun,CUI Dafang.Genetic Relationships Among 11 Species of Medicago and Relative Species Using Microsatellite (SSR and ISSR) Markers[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2014,53(1). | |
| Authors: | LI Feifei YANG Haijun CUI Dafang |
| Institution: | 1. College of Life and Environmental Science,Central University for Nationalities,Beijing 100081,China; 2. Center of Experimental Teaching for Common Basic Courses, South China Agricultural University, Guangzhou 510642, China; 3. College of Forestry, South China Agricultural University, Guangzhou 510642, China |
| Abstract: | The genetic relationships among 11 species of Medicago and relative species were studied by using 30 pair of SSR primers and 12 ISSR primers. Based on genetic diversity analysis and UPGMA cluster analysis the results showed that 4 species of subgen. Platycapos were clustered together, related to their similar morphological characteristics; species of subgen. Platycapos were clustered with Trigonella, while T. cancellata were nested in subgen. Platycapos clade; the genetic distances among 3species of M. sativa complex were shortest; M. polymorpha was closer to Trigonella in ISSR results. Thus Trigonella had close genetic relationship with Medicago, especially species of subgen. Platycapos, and relatively farther from other species in Medicago. |
| Keywords: | Medicago relative species microsatellite markers genetic relationships |
| 本文献已被 CNKI 等数据库收录！ | |
| 点击此处可从《中山大学学报(自然科学版)》浏览原始摘要信息 | |
| 点击此处可从《中山大学学报(自然科学版)》下载免费的PDF全文 | |