白蚁肠道木质素分解菌PY12的分离鉴定及lip基因的克隆

利用苯胺蓝平板法从白蚁肠道中分离到一株木质素分解高效菌株PY 12,经形态观察、生化鉴定和16 SrRNA鉴定为肠杆菌属的霍氏肠杆菌(Enterobacterhormaechei).根据已报道的lip(木质素过氧化物酶,Lignin Peroxidase)基因序列设计特异性引物,克隆该菌的lip基因,将其克隆入pMD19-T载体,获得的核苷酸序列为918 bp,并与GenBank中已知的lip序列进行同源性对比,同源性不高,但该核苷酸翻译后的氨基酸序列比对结果发现,其与假单胞杆菌Pseudomonas sp.编码蛋白质的氨基酸序列同源性为88.5%.

Isolation and identification of lignin decomposing bacteria and cloning of lip gene in termite gut

A strain of bacterium that named PY12 was isolated from
the termite gut using Azure B method, which had an efficiently
lignin decomposition function. It was identificated as
\textit{Enterobacter hormaechei}. by morphological, biochemical
identification and\\ 16S rRNA identification. The $lip$ gene were
amplified by the specific primers, and then cloning it into pMD19-T
vector, the target fragment sequences were 918 bp, Homology
comparison with the $lip$ gene which known in GenBank, the Homology
was low, but when the nucleotide sequences were translated into
protein, the homology was up to 88.5{\%} with the Pseudomonas sp..