槐米中黄酮类成分的超声提取及RP-HPLC色谱分析

运用反相高效液相（RP-HPLC）色谱法测定槐米中芦丁、槲皮素等黄酮类化合物．采用InertSustain C18色谱柱，柱温25℃，甲醇︰水=70︰30为流动相，流速0.8 mL/min 等度洗脱，检测波长254nm.实验结果表明，各组分的色谱峰均达到基线分离，该方法重复性好，灵敏度高，回收率高．对超声提取和热碱提取-酸沉淀提取的样品前处理方法进行比较，采用HPLC法考察不同前处理方法对提取成分及含量的影响，结果表明，超声提取无需加热，比热碱提取-酸沉淀法提取时间短，面积归一法分析超声60%乙醇提取产物中芦丁占98.6%，未检测到槲皮素.

Analysis of Flavonoids in Flos Sophorae Ultrasonic Extraction and RP-HPLC Chromatography

A reversed-phase high performance liquid chromatography （RP-HPLC） method for the separation and determination of flavonoids such as rutin and quercetin was developed. The extract was separated using InertSustain C18 column by isocratic elution with MeOH-H 2 O （70︰30, V/V） as mobile phase at a flow of 0.8mL/min and detection wavelength was set at 254 nm. The results showed that each component was baseline separated; high sensitivity and recovery rate were acceptable. Two methods including ultrasonic-assisted extraction and alkali extraction and acid precipitation were compared, the impaction of different sample preparation on the content was determined by HPLC in this work. The experimental results showed that ultrasonic extraction gave a significant reduction in extraction time in room temperature, HPLC analysis showed that 98.6%rutin, but quercetin was not detected in the ultrasonic extract.