| 重组腺相关病毒质量控制的qPCR技术研究进展 |
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| 引用本文： | 肖桂清,杨会勇,刁勇.重组腺相关病毒质量控制的qPCR技术研究进展[J].华侨大学学报(自然科学版),2014,0(2):191-195. |
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| 作者姓名： | 肖桂清 杨会勇 刁勇 |
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| 作者单位： | 华侨大学 分子药物研究院, 福建 泉州 362021 |
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| 基金项目： | 国家自然科学基金资助项目(81371669,81271691);国家国际科技合作项目(2011DFG33320) |
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| 摘 要： | 综述实时荧光定量PCR(qPCR)技术在重组腺相关病毒(rAAV)的基因组滴度测定中的应用成果,以及qPCR技术在rAAV质量控制过程中的应用,如感染滴度及rcAAV污染率的测定等.分析qPCR技术存在的适用范围窄、易产生系统性误差和易受rAAV杂质干扰等问题的原因,并探讨相关的解决策略.
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| 关 键 词： | 重组腺相关病毒 基因组滴度 实时荧光定量PCR 基因治疗 |
Advances in Real-Time Quantitative PCR Technology for Quality Control of Recombinant Adeno-Associated Virus |
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| XIAO Gui-qing;YANG Hui-yong;DIAO Yong.Advances in Real-Time Quantitative PCR Technology for Quality Control of Recombinant Adeno-Associated Virus[J].Journal of Huaqiao University(Natural Science),2014,0(2):191-195. |
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| Authors: | XIAO Gui-qing;YANG Hui-yong;DIAO Yong |
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| Institution: | Institute of Molecular Medicine, Huaqiao University, 362021 Quanzhou, China |
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| Abstract: | This paper reviews the application achievement of real-time quantitative PCR(qPCR)technology in the recombinant adeno-associated virus genome titer determination and its application in rAAVs’ quality control, including infectious titer and contamination rate of rcAAV. The current several problems of qPCR technique, such as relatively narrow application range, easy to cause systemic errors and be interfered by rAAV impurity, are analyzed and discussed on better countermeasures. |
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| Keywords: | recombinant adeno-associated virus genome titration real-time quantitative PCR gene therapy |
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