| 稀土离子对TaqTMDNA聚合酶的抑制机理 |
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| 引用本文： | 黄德盈,蔡玺,吴士筠,杉浦幸雄.稀土离子对TaqTMDNA聚合酶的抑制机理[J].中南民族大学学报(自然科学版),2003,22(2):1-4,16. |
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| 作者姓名： | 黄德盈 蔡玺 吴士筠 杉浦幸雄 |
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| 作者单位： | [1]CollegeofChemandLifeScience,SCUFN [2]InstituteforChemicalChemicalResearch,KyotoUniversity,UjiCity,Japan611 |
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| 基金项目： | 国家民委科研基金资助项目 (MZY980 0 1 ) |
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| 摘 要： | 在PCR扩增水平上研究了La^ 3和Ce^ 3对Taq^TMDNA聚合酶的抑制机理,采用动态荧光比色法测定PCR扩增产物浓度，证明了La^ 3和Ce^ 3在10～50μmol／L抑制DNA复制是由于其抑制了Taq^TMDNA聚合酶的活性所致,且该抑制属竞争性的抑制，得到了La^ 3和Ce^ 3对Taq^TMDNA聚合酶的抑制常数分别为12．7μmol／L和14．4μmol／L。
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| 关 键 词： | 稀土离子 PCR扩增 Taq DNA聚合酶 |
Inhibitory Mechanism of Trivalent Rare-Earth Ions on TaqTM DNA Polymerase |
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| HUANG Deying,Cai xi,Wu Shijun,Yukio sugiura.Inhibitory Mechanism of Trivalent Rare-Earth Ions on TaqTM DNA Polymerase[J].Journal of South-Central Univ for,2003,22(2):1-4,16. |
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| Authors: | HUANG Deying Cai xi Wu Shijun Yukio sugiura |
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| Abstract: | The mechanism of inhibitory of La3+ and Ce3+ on active TaqTM DNA polymerase was investigated by PCR amplification. The concentration of PCR amplification product DNA was measured with the kinetic fluorescence spectrum. The concentrations of La3+ and Ce3+, which inhibited the DNA replication were between 10 and 50 μmol/L. The mechanism of inhibition to the DNA replication by La3+ and Ce3+ was found that both the trivalent ions inhibited the activity of TaqTM DNA polymerase via a competitive inhibition during the PCR amplification proceedings. The inhibition constants were measured to be 12.7 μmol/L and 14.4 μmol/L for La3+ and Ce3+, respectively. |
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| Keywords: | rare earth metal ions PCR amplification Taq TM DNA polymerase |
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