美洲大蠊药材质量控制方法的研究

目的:制定美洲大蠊药材的鉴别、检查及含量测定方法.方法:采用薄层色谱法定性鉴别,色谱条件为正丁醇∶乙酸∶水(4∶1∶1),0.5%茚三酮显色.分光光度法进行总氨基酸的含量测定,茚三酮显色后检测波长为570nm.采用水分测定法检查水分的最低控制限为12%.采用热浸法检查乙醇浸出物的最低控制限为10%.结果:薄层色谱鉴别斑点清晰,易于识别,分光光度法测定总氨基酸含量,回归方程:A=0.067943C 0.009143,r=0.9999,酪氨酸含量在1.0～10.0μg范围内呈良好的线性关系.结论:该方法简便易行,重现性好,可用于美洲大蠊药材的质量控制.

Studies of quality control method on Periplaneta americana

Objective: To study the methods of identification and assay in Periplanta americana.Methods: TLC was used and the chromatographic condition was as follows: n-Butanol,Aceticacid and water(4:1:1);when determining the content of total amino acids by pectrophotometry after ninhydrin colouring,the determination wavelength was 570nm in ninhydrin colouring.The minimum control limit of the water content was 12% by using water content assay method.The content of ethanol soluble extraction should be no less than 10% by hot dip.Result: The method of TLC was exclusive and spots were clear,the determination of amino acid by spectrophotometry,regression equation is A=0.067943C 0.009143,r=0.9999,Tyrosine content varied from 1.0 to 10.0 g and the linearity is good.Conclusion: The methods are simple and accurate.The method can be used for the quality control of Periplanta americana.