| 胃癌中p53基因失活及其蛋白产物的免疫组化分析 |
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| 引用本文： | 吴国俊,李明发.胃癌中p53基因失活及其蛋白产物的免疫组化分析[J].复旦学报(自然科学版),1998,37(2):135-140. |
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| 作者姓名： | 吴国俊 李明发 |
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| 作者单位： | 复旦大学(吴国俊，郑其平，余龙，赵寿元)，南京铁道医学院(李明发，黄培林，单祥年) |
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| 基金项目： | 国家自然科学基金，江苏省自然科学基金，江苏省优秀青年教师基金 |
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| 摘 要： | 应用ＰＣＲ－ＳＳＣＰ银染，Ｓｏｕｔｈｅｒｎ杂我及免疫组化等方法同步研究２５例胃癌标本的ｐ５３基因突变，杂合性丢失及蛋白持表达，在２２例胃癌中同时获得有关ｐ５３基因突变和杂合性丢失的检测结果，被检出ｐ５３基因突变的５例胃癌中，２例伴有杂合性丢失，３例仅有ｐ５３基因突变。
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| 关 键 词： | 胃癌 P53基因 失活 杂合性丢失 免疫组织化学 |
Study on Inactivation of p53 Gene and Immunohistochemical Analysis of p53 Protein in Gastric Cancer |
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| Wu Guojun, Li Mingfa, Zheng Qiping, Huang Peilin, Yu Long, Shan Xiangnian, Zhao Shouyuan.Study on Inactivation of p53 Gene and Immunohistochemical Analysis of p53 Protein in Gastric Cancer[J].Journal of Fudan University(Natural Science),1998,37(2):135-140. |
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| Authors: | Wu Guojun Li Mingfa Zheng Qiping Huang Peilin Yu Long Shan Xiangnian Zhao Shouyuan |
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| Abstract: | Matation, loss of heterozygosity (LOH) and overexpression of p53 gene were simultaneously examined in 25 cases of gastric cancer using PCR-SSCP silver-staining, southern blot and immunohistochemistry. The results of p53 gene mutation and LOH were found in 22 cases of gastric cancer. In 5 cases of gastic cancer with p53 gene mutation, LOH was found in only 2 cases. This result indicated there were at least 2 types of inactivition of p53 in gastric cancer. Comparative analysis of p53 gene mutation and the overexpression of p53 gene in 15 cases indicated the overexpression of p53 gene was closely related with p53 gene mutation in gastric cancer. In addition, LOH was not found in 3 cases with p53 overexpression. This result supported the hypothesis that the dominant negtive effect of p53 mutant existed in gastric cancer. |
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| Keywords: | gastric cancer p53 gene gene inactivation LOH immunohistochemistry |
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