| 液相色谱法测定玉米乳酸发酵饮料的糖含量 |
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| 引用本文： | 杨本宏,郑敏,陈玉光.液相色谱法测定玉米乳酸发酵饮料的糖含量[J].合肥工业大学学报(自然科学版),2002,25(1):147-149. |
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| 作者姓名： | 杨本宏 郑敏 陈玉光 |
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| 作者单位： | 合肥联合大学化学生物工程系,安徽,合肥,230022 |
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| 摘 要： | 采用液相色谱法分析测定玉米粉乳酸发酵饮料中的糖含量 ,以 Hypersil-NH2 为分析柱 ,乙腈 -水为流动相 ,利用示差折光检测器 ,可以将饮料中果糖、葡萄糖、乳糖和多种低聚麦芽糖较好地分离 ,并测定其各自的含量 ,所得结果良好 ,变异系数为 0 .81%～ 2 .5 % ,线性相关系数为 0 .9996～ 0 .9999,回收率为 97.0 %～ 98.5 %。
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| 关 键 词： | 液相色谱法 玉米乳酸饮料 糖含量 |
| 文章编号： | 1003-5060(2002)01-0147-03 |
| 修稿时间： | 2001年1月3日 |
Determination of the sugar content in corn beverage fermented by lactic acid bacteria by means of liquid chromatography |
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| YANG Ben hong,ZHENG Min,CHEN Yu guang.Determination of the sugar content in corn beverage fermented by lactic acid bacteria by means of liquid chromatography[J].Journal of Hefei University of Technology(Natural Science),2002,25(1):147-149. |
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| Authors: | YANG Ben hong ZHENG Min CHEN Yu guang |
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| Abstract: | The method of liquid chromatography is applied to determine the sugar type and content in corn beverage fermented by lactic acid bacteria. With the column of Hypersil NH 2, the mobile phase of acetonitrile water and a differential index detector, various sugar in corn beverage, such as fructose, glucose, lactose and several maltooligoscchrides, were effectively separated, and the corresponding contents were also determined respectively. The coefficients of variation of the determination were 0.81%~2.5%, the linearly dependent coefficients were 0.999 6~0.999 9, and the recovery rates were 97.0%~98.5%. |
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| Keywords: | liquid chromatography corn lactic acid beverage sugar content |
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