| 观赏与食用两用型羽衣甘蓝的组培快繁试验 |
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| 引用本文： | 曹君迈,陈彦云.观赏与食用两用型羽衣甘蓝的组培快繁试验[J].河南科技大学学报(自然科学版),2006,27(4):63-65. |
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| 作者姓名： | 曹君迈 陈彦云 |
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| 作者单位： | 1. 西北第二民族学院,生命科学系,宁夏,银川,750021
2. 宁夏大学,生命科学学院,宁夏,银川,750021 |
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| 基金项目： | 宁夏教育厅基金项目(2001103) |
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| 摘 要： | 研究了观赏与食用型羽衣甘蓝的组培快繁技术,结果表明:适宜的起始分化培养基为MS BA0.7mg/L NAA0.1mg/L 30g/L蔗糖,25d可分化出小苗,分化率达93%;适宜的增殖培养基为MS BA2mg/L NAA0.02mg/L 30g/L蔗糖,繁殖系数为4.5;适宜的生根培养基为MS NAA0.2mg/L IBA0.8mg/L 20g/L蔗糖,10d左右可长出3~4条新根,生根率90%,移栽成活率达90%以上。
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| 关 键 词： | 羽衣甘蓝 组培快繁技术 培养基 分化率 繁殖系数 |
| 文章编号： | 1672-6871（2006）04-0063-03 |
| 收稿时间： | 2005-09-28 |
| 修稿时间： | 2005年9月28日 |
Research of Fast Multiplication for the Tissue Culture of Brassica Oleracea Var Acephala Both for Sight and Food |
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| CAO Jun-Mai,CHEN Yan-Yun.Research of Fast Multiplication for the Tissue Culture of Brassica Oleracea Var Acephala Both for Sight and Food[J].Journal of Henan University of Science & Technology:Natural Science,2006,27(4):63-65. |
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| Authors: | CAO Jun-Mai CHEN Yan-Yun |
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| Institution: | CAO Jun-Mai~1,CHEN Yan-Yun~2 |
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| Abstract: | The techniques of fast multiplication for the tissue culture of Brassica Oleracea Var Acephala both for sight and food were researched.The results shows that the suitable medium for starting differentiation is MS BA0.7mg/L NAA0.1mg/L 30g/L sucrose.Little seedlings are differentiated on the 25th day with differentiation rate of 93%.The suitable medium for multiplication is MS BA2mg/L NAA0.02mg/L 30g/L sucrose.The propagation coefficient is 4.5.The suitable medium for rooting is MS NAA0.2mg/L IBA0.8mg/L 20g/L sucrose.3-4 new roots grow on about the 10th day with rooting rate of over 90%.The survival rate of transplantation reaches over 90%. |
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| Keywords: | Brassica Oleracea Var Acephala Techniques of fast multiplication for the tissue culture Medium Differentiation rate Propagation coefficient |
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