Evaluating the reliability of microsatellite genotyping from low-quality DNA templates with a polynomial distribution model |
| |
Authors: | Gang He Kang Huang SongTao Guo WeiHong Ji XiaoGuang Qi Yi Ren XueLin Jin BaoGuo Li |
| |
Affiliation: | (1) Key Laboratory of Resource Biology and Biotechnology in Western China of Ministry of Education, College of Life Sciences, Northwest University, Xi’an, 710069, China;(2) Ecology &; Conservation Group, Institute for Natural Sciences, Massey University, Auckland, 904, New Zealand;(3) Institute of Zoology, Shaanxi Academy of Sciences, Xi’an, 710032, China;(4) Shaanxi Wild Animal Rescue and Research Center, Xi’an, 710402, China; |
| |
Abstract: | Molecular studies using trace DNA, such as from museum specimens, ancient or forensic samples and samples obtained noninva- sively, often have a common problem of low quality of DNA templates. Amplification errors, such as allelic dropout and false allele, may arise during polymerase chain reaction (PCR) using such samples. A mathematical model which treats homozygotes and heterozygotes discriminately has been developed to measure sample quality and compute the confidence level of using multiple- tube appro... |
| |
Keywords: | Sichuan snub-nosed monkey noninvasive sampling microsatellite genotyping errors polynomial distribution model |
本文献已被 CNKI 维普 SpringerLink 等数据库收录! |
|