Integration of rolling circle amplification and cationic conjugated polymer for the homogeneous detection of single nucleotide polymorphisms |
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| Authors: | ZhiYuan Tang YongQiang Cheng Qing Du HongXia Zhang ZhengPing Li |
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| Affiliation: | TANG ZhiYuan,CHENG YongQiang *,DU Qing,ZHANG HongXia & LI ZhengPing * Key Laboratory of Medicine Chemistry and Molecular Diagnosis,Ministry of Education,College of Chemistry and Environment Science,Hebei University,Baoding 071002,China |
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| Abstract: | A novel, homogeneous and sensitive assay for the detection of single nucleotide polymorphisms (SNPs) by integration of rolling circle amplification (RCA) and cationic conjugated polymer (CCP) has been developed and tested. Mutant DNA serves as the template for specifically circularizing a padlock probe (PLP) with a sequence that is complementary to the mutant DNA. Afterwards, the mutant DNA directly acts as the primer to initiate the RCA reaction in the presence of phi29 DNA polymerase that generates a long... |
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| Keywords: | rolling circle amplification cationic conjugated polymer single nucleotide polymorphisms homogeneous detection |
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