| 水稻OsRacD与OsRhoGDI2基因共表达载体的构建 |
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| 引用本文： | 石宏浩,靳萍,王利娜,梁卫红.水稻OsRacD与OsRhoGDI2基因共表达载体的构建[J].河南师范大学学报(自然科学版),2012,40(4):113-115. |
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| 作者姓名： | 石宏浩 靳萍 王利娜 梁卫红 |
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| 作者单位： | 河南师范大学生命科学学院,河南新乡,453007 |
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| 基金项目： | 国家自然科学基金面上项目，教育部科学技术研究重点项目，河南省科技创新杰出青年计划，河南省教育厅自然科学研究计划项目 |
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| 摘 要： | 水稻OsRacD是从水稻幼穗中分离的Rho基因,OsRhoGDI2是通过酵母双杂交筛选到的与OsRacD相互作用蛋白的编码基因,已有的研究表明OsRacD与OsRhoGDI2之间可能存在相互作用,并参与水稻育性调控过程.为了鉴定二者的相互作用,本研究构建了OsRacD与OsRhoGDI2基因共表达载体,为采用免疫共沉淀验证二者在体内的相互作用,解析OsRacD与OsRhoGDI2基因参与水稻育性控制的分子机制奠定了基础.
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| 关 键 词： | 水稻 OsRacD基因 OsRhoGDI2基因 共表达载体 |
Construction of the Co-expresstion Vector of Rice OsRhoGDI2 and OsRacD Gene |
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| SHI Hong-hao
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JIN Ping
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WANG Li-na
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LIANG Wei-hong.Construction of the Co-expresstion Vector of Rice OsRhoGDI2 and OsRacD Gene[J].Journal of Henan Normal University(Natural Science),2012,40(4):113-115. |
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| Authors: | SHI Hong-hao JIN Ping WANG Li-na LIANG Wei-hong |
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| Institution: | (College of Life Sciences,Henan Normal University,Xinxiang 453007,China) |
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| Abstract: | OsRacD gene was isolated from panicle as one member of rice Rho family,and its putative partner OsRhoGDI2 gene was further obtained by yeast two-hybrid screening.Our previous study showed that OsRacD and OsRhoGDI2 may participate the rice fertility control by protein-protein interactions.In order to investigate their interactions in vivo,the co-expression vector of OsRacD and OsRhoGDI2 was constructed in this paper which facilitated the binding analysis with co-immunoprecipitation,and also helped to resolve their underlying molecular mechanism in rice development and fertility control. |
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| Keywords: | Rice OsRacD gene OsRhoGDI2 gene co-expresstion vector |
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