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| 阿魏酸与血清蛋白相互作用研究 | |
| 引用本文： | 王玲,赵东旺,訾丽娟,毛烨炫,刘艳菊. 阿魏酸与血清蛋白相互作用研究[J]. 河南师范大学学报(自然科学版), 2012, 40(4): 87-90 |
| 作者姓名： | 王玲 赵东旺 訾丽娟 毛烨炫 刘艳菊 |
| 作者单位： | 1. 郑州师范学院化学系,郑州,450044 2. 郑州大学化学系,郑州,450001 3. 河南中医学院药学院,郑州,450008 |
| 基金项目： | 河南省科技攻关项目，河南省教育厅科学技术项目 |
| 摘 要： | 应用紫外-可见吸收光谱及荧光光谱法研究了阿魏酸与牛血清蛋白(BSA)在1∶1甲醇水体系中的相互作用.结果表明阿魏酸与BSA发生反应生成新的复合物,荧光猝灭机制为静态猝灭;根据能量转移原理求得阿魏酸与BSA间的结合距离为4.41nm.进一步考察金属离子对此结合反应的影响,结果表明金属离子存在时,BSA荧光猝灭程度加大. |
| 关 键 词： | 紫外-可见吸收光谱 荧光光谱 阿魏酸 牛血清蛋白 |
Investigations of the interaction between Ferulic Acid and Serum Albumin |
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| WANG Ling , ZHAO Dong-wang , ZI Li-juan , MAO Ye-xuan , LIU Yan-ju. Investigations of the interaction between Ferulic Acid and Serum Albumin[J]. Journal of Henan Normal University(Natural Science), 2012, 40(4): 87-90 | |
| Authors: | WANG Ling ZHAO Dong-wang ZI Li-juan MAO Ye-xuan LIU Yan-ju |
| Affiliation: | 1.Department of Chemistry,Zhengzhou normal University,Zhengzhou 450044,China;2.Department of Chemistry,Zhengzhou University, Zhengzhou 450001,China;3.Pharmacy College,Henan University of Traditional Chinese Medicine,Zhengzhou 450008,China) |
| Abstract: | In this paper the interaction between Ferulic acid and bovine serum albumin(BSA) in 1∶1 CH3OH-H2O was studied by Fluorescence spectroscopic technique and UV-Vis absorption Spectroscopy in this paper.The results indicated that Ferulic acid could form non-covalent complexes with BSA and the quenching mechanism was static quenching.The distance between them was 4.41 nm according to the theory of the Frster energy transference.Furtherly,the effection of different metals to this interaction was explored,which showed that the quenching degree of fluorescence was aggravated in the presence of metals. |
| Keywords: | UV-Vis absorption spectroscopy fluorescence spectroscopy ferulic acid bovine serum albumin |
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