X射线辐射对仔鼠肝组织结构和相关蛋白的影响

 为探讨X射线辐射对仔鼠肝重量、肝组织结构及肝中Bax蛋白表达的影响，对160只仔鼠（出生6~7d），用不同剂量（0、1、3、5、7Gy）的X射线进行全身辐射，分别于辐射后1、5、10、20d，检测各期仔鼠肝重和体重的变化，用显微技术观察仔鼠肝脏组织结构的变化，用免疫组织化学方法检测肝脏中Bax蛋白的表达。结果表明，X射线辐射影响仔鼠肝脏的生长发育，辐射后5～20d，辐射组仔鼠肝重均低于对照组，差异显著（P<0.05）或极显著（P<0.01）；除1Gy辐射组外，其他辐射组在5～20d时仔鼠体重均低于对照组，差异极显著（P<0.01）；随辐射剂量增大，辐射组仔鼠肝中Bax蛋白阳性表达逐渐增强；辐射后仔鼠肝小叶结构不清，肝板变细，肝血窦变大，肝细胞变形，排列不规则，细胞核萎缩，细胞出现空泡化。

Effects of X-ray on Histological Structure and Interrelated Polypeptides in Liver of Filial Mice

To explore the effects of X-ray on weight，histological structure and expression of Bax protein in liver of developing filial mice,160 mice of 6～7 days after birth were radiated with different dosages (0, 1, 3, 5, 7Gy) of X-ray. Changes of liver and body weight were examined, histological structure was observed using bio-microscopy，and the expression of Bax protein was measured by immunohistochemical staining in filial mice liver at 1, 5, 10 and 20d after irradiation. Results show that X-ray influences the growth and development of the filial mice liver. The liver weight of filial mice of the experimental groups is lower than that of the control group 5~20d after irradiation (P<0.05 or P<0.01), except experimental groups with 1Gy; the body weight of filial mice of the experimental groups is lower than that of the control group 5~20d after irradiation(P<0.01); the expression intensity of Bax protein in the liver of filial mice is significantly increased with the increase of the dose; the structure of hepatic lobule is unclear, hepatic plate is thinner, hepatic sinusoid is stronger, accompanied with hepatocyte disfiguration, irregular arrangement, nucleus atrophy, and vacuolization, after irradiation.