LSA-10型大孔树脂分离恒山黄芪总黄酮的吸附动力学

为探索LSA-10型树脂对于恒山黄芪总黄酮的吸附特性以及分离工艺.通过7种树脂的静态吸附解吸实验,确定大孔吸附树脂的选型,考察吸附动力学、吸附等温线,并确定该树脂分离黄酮的工艺.吸附动力学研究表明,吸附过程拟二阶模型比拟一阶模型能更好拟合LSA-10型树脂的吸附过程;吸附等温线研究表明,LSA-10型树脂对黄芪总黄酮的吸附最符合Langmuir等温吸附方程;LSA-10型大孔吸附树脂对黄芪中总黄酮分离的最佳条件:黄芪提取液的上样浓度0.5 mg/mL,上样体积为4 BV(床体积),上样流速3 mL/min,以8 BV、60％乙醇以2 BV/h的速度进行洗脱.LSA-10树脂对黄芪总黄酮的吸附能力显著大于其他成分,这种吸附能力的差异与验证实验相吻合.

Kinetics of total flavonoids in Astragalus Membranaceus sepatated by LSA-10

Objective: Study on adsorption characteristics and optimum extraction technology of total flavonoids from Astragalus membranaceus by LSA-10 Resin. Methods: Through the static adsorption and desorption experiments of 7 kinds of resins, the selection of macroporous adsorption resins was determined, the adsorption kinetics and adsorption isotherms were investigated. The process of flavonoid separation by this resin was optimized. Results: Kinetic studies show that the pseudo-second-order model of the adsorption process can better fit the adsorption process of the LSA-10 resin than the pseudo-first-order model. The adsorption isotherm studies show that the adsorption of the total flavones of astragalus by the LSA-10 resin is most consistent with the Langmuir isotherm adsorption equation. Optimal conditions for the separation of total flavonoids in Astragalus membranaceus by LSA-10 macroporous adsorption resin is: loading concentration of astragalus extract 0.5 mg/mL, loading volume 4 BV(bed volume), loading flow rate 3 mL/min, eluent dosage 8 BV, 60 % ethanol was eluted at a rate of 2 BV/h. Conclusion: The adsorption capacity of LSA-10 resin for the total flavonoids of Astragalus Membranaceus was significantly greater than that of other components.