亲水/疏水半互穿网凝胶对牛血清白蛋白的控制释放

以牛血清白蛋白为对象,研究了亲水/疏水(QPVPD/PAAc)半互穿网凝胶对牛血清白蛋白的吸附与释放过程.在吸附过程中,当溶液pH值在牛血清白蛋白等电点(4.7)附近时,凝胶对蛋白质的吸附值最大;而在释放过程中,pH=7的释放率远大于其它pH值时的释放率.实验表明凝胶结构对蛋白质吸附与释放有较大影响.随着疏水成分的增加,凝胶对牛血清白蛋白的释放速率变缓.

Controlled release of bovine serum albumin in hydrophilic-hydrophobic semi-IPN hydrogel

Hydrophilic-hydrophobic semi-IPN hydrogels were prepared. The absorption and release of protein on polysoap gels were investigated using bovine serum albumin as a model molecule. There are two processes during the absorption: diffusion of protein into the gels and conjugate of protein on the gels. The maximum absorption was observed when the pH of solutions was near the isoelectric point of protein. A larger release ratio was observed at pH 7. The results show that the diffusion of protein into the gels and interaction of protein on the gels play an important role during the process of absorption or release. The release ratio of bovine serum albumin becomes slowly with the increasing of hydrophobic interaction in the gel.