| 天花粉蛋白抗人免疫缺陷病毒1型研究 |
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| 引用本文： | 赵巧云,黎志东,宋纪蓉. 天花粉蛋白抗人免疫缺陷病毒1型研究[J]. 西北大学学报(自然科学版), 2006, 36(1): 85-88 |
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| 作者姓名： | 赵巧云 黎志东 宋纪蓉 |
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| 作者单位： | 西北大学化工学院 陕西西安710069(赵巧云，宋纪蓉)，第四军医大学微生物学教研室 陕西西安710032(黎志东) |
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| 基金项目： | 陕西省科技攻关计划基金资助项目(2003K10G9)，西安市科技攻关计划基金资助项目(GG05080) |
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| 摘 要： | 目的检测用基因工程方法制备的天花粉蛋白抗人免疫缺陷病毒1型的活性。方法将人免疫缺陷病毒1型在细胞系中培养,加入天花粉蛋白后,观察细胞病变情况,检测P24抗原及逆转录酶的活性。结果加入天花粉蛋白的细胞始终未见细胞病变,P24抗原及逆转录酶检测均为阴性;阴性对照组细胞在第7 d以后均出现人免疫缺陷病毒1型感染典型细胞病变,P24抗原及逆转录酶检测均为阳性;阳性对照组始终未见HIV-1感染典型细胞病变,逆转录酶的活性检测和P24抗原检测结果均为阴性。结论天花粉蛋白在培养细胞系中可以有效地抑制人免疫缺陷病毒1型。
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| 关 键 词： | 天花粉蛋白 人免疫缺陷病毒1型 抑制 |
| 文章编号： | 1000-274X（2006）01-0085-04 |
| 收稿时间： | 2004-10-11 |
| 修稿时间： | 2004-10-11 |
Effect of trichosanthin on suppressing human immunodeficiency virus 1 activity |
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| ZHAO Qiao-yun,LI Zhi-dong,Song Ji-rong. Effect of trichosanthin on suppressing human immunodeficiency virus 1 activity[J]. Journal of Northwest University(Natural Science Edition), 2006, 36(1): 85-88 |
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| Authors: | ZHAO Qiao-yun LI Zhi-dong Song Ji-rong |
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| Abstract: | Aim To detect the anti-human immunodeficiency virus activity of trichosanthin expressed by the means of biotechnology.Methods Trichosanthin were put into the cells infected by HIV1 and the activity of HIV P24 antigen and RT activity were detected.Results It was found that the cells added into trichosanthin showed RT activity negative,P24 Ag negative and no representative cell pathomorphology change while the cells added into distilled water showed RT activity positive,P24 Ag positive and representative cell pathomorphology changed,and the cells added into zidovudine showed RT activity negative,P24 Ag negative and no representative cell pathomorphology change.Conclusion Trichosanthin can inhibit human immunodeficiency virus 1 in vitro. |
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| Keywords: | trichosanthin human immunodeficiency virus 1 inhibition |
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