Construction and<Emphasis Type="Italic">in vitro</Emphasis> study of an E1B-defective adenovirus |
| |
Authors: | Xue?Feng Joshua?Mallam?Nock Zhu?Hua-bin Dong?Chang-yuan Email author" target="_blank">Qi?Yi-pengEmail author |
| |
Institution: | (1) Institute of Virology, Wuhan University, 430072 Wuhan, Hubei, China |
| |
Abstract: | An E1B-defective adenovirus named r1/Ad was constructed by homologous recombination. The construction, selection and propagation
of recombinant virus was done in the human embryonic kidney 293 cells (HEK293). Thein vitro study demonstrated that the recombinant virus has the ability to replicate in and lyse some p53-deficient human tumor cells
such as the human glioblastoma tumor cells (U251) and human bladder tumer cells (EJ) but not in the normal cells with functional
p53 such as the human fibroblast cells (MRC-5). Also, based on the cytopathic effect (CPE), it was demonstrated that the U251
cells were more sensitive to the infection of r1/Ad than that of EJ cells under identical conditions. In this paper, it was
found that r1/Ad could be very useful in studying thein vitro selective replication of E1B-defective adenovirus. This may help to determine the safety of using any E1B-defective adenoviruses
in cancer gene therapy.
Foundation item: Supported by the National Natural Science Foundation of China (3988003)
Biography: Xue Feng (1978-), male, Master, research direction: cancer gene therapy by recombinant virus. |
| |
Keywords: | E1B-defective adenovirus cytopathic effect cancer gene therapy |
本文献已被 维普 SpringerLink 等数据库收录! |
|