| LA-PCR法制备赤麂Y2涂染探针与原位杂交 |
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| 引用本文： | 粘伟红,潘丹岷,曹祥荣,张锡然. LA-PCR法制备赤麂Y2涂染探针与原位杂交[J]. 南京师大学报(自然科学版), 2006, 29(2): 78-80 |
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| 作者姓名： | 粘伟红 潘丹岷 曹祥荣 张锡然 |
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| 作者单位： | 南京师范大学生命科学学院,江苏,南京,210097;南京师范大学生命科学学院,江苏,南京,210097;南京师范大学生命科学学院,江苏,南京,210097;南京师范大学生命科学学院,江苏,南京,210097 |
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| 摘 要： | 应用显微切割技术获得赤麂的Y2单条染色体,经LA-PCR（linker-adaptor PCR）扩增后用DIG标记制备探针,然后用Southern blot杂交法对所制备的探针进行验证并对毛冠鹿的中期核型进行原位杂交.结果表明：用该法制备的涂染探针是成功的,原位杂交也获得了阳性结果,可以初步验证毛冠鹿中的Y染色体.
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| 关 键 词： | LA-PCR Southern杂交 原位杂交 赤麂 毛冠鹿 |
| 文章编号： | 1001-4616（2006）02-0078-03 |
| 收稿时间： | 2005-06-20 |
| 修稿时间： | 2005-06-20 |
Label Y2 Paint Probes of Munticus Muntjac Vaginalis by LA-PCR and in situ Hybridization |
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| Nian Weihong,Pan Danmin,Cao Xiangrong,Zhang Xiran. Label Y2 Paint Probes of Munticus Muntjac Vaginalis by LA-PCR and in situ Hybridization[J]. Journal of Nanjing Normal University(Natural Science Edition), 2006, 29(2): 78-80 |
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| Authors: | Nian Weihong Pan Danmin Cao Xiangrong Zhang Xiran |
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| Affiliation: | School of Life Science, Nanjing Normal University, Nanjing 210097, China |
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| Abstract: | Y_2 chromosome of M.m vaginalis is obtained by microdissection technology,and probes are made with DIG labels after the DNA copies by LA-PCR(linker-adaptor PCR)are amplified.Labeled probes are detected by Southern blot hybridization,and in situ hybridization of the metaphase karyotype of Elaphodus cephalophus are made.The results indicate that it is successfully to make the paint probe with the method,and masculine results are also obtained in the in situ hybridization,and thus the Y chromosome of Elaphodus cephalophus can be proved. |
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| Keywords: | LA-PCR |
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