| 中国美利奴细毛羊BAC文库的质量评价 |
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| 引用本文： | 詹勇华,陈创夫,高剑峰,王金富.中国美利奴细毛羊BAC文库的质量评价[J].石河子大学学报,2006,24(1):102-104. |
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| 作者姓名： | 詹勇华 陈创夫 高剑峰 王金富 |
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| 作者单位： | [1]新疆地方与民族高发病实验室,新疆石河子832003 [2]石河子大学生命科学学院,新疆石河子832003 |
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| 摘 要： | 从文库的平均插入片段大小、文库的覆盖率、文库克隆的稳定性对中国美利奴细毛羊BAC文库进行了质量评价.统计结果表明,文库的平均插入片段大小约为133kb, 非重组克隆占3.2%,文库在理论上约有8倍绵羊基因组覆盖率,从该文库中找到任意单拷贝序列的概率为99.93%.连续多次传代培养实验证实文库克隆的遗传稳定性好.
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| 关 键 词： | BAC文库 质量评价 绵羊基因组 遗传稳定性 |
| 文章编号： | 1007-7383（2006）01-0102-03 |
| 收稿时间： | 2005-11-09 |
| 修稿时间： | 2005年11月9日 |
Quality Assessment of BAC Library of Chinese Fine-wool Merino Sheep |
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| ZHAN Yong-hua,CHEN Chuang-fu,GAO Jian-feng,WANG Jin-fu.Quality Assessment of BAC Library of Chinese Fine-wool Merino Sheep[J].Journal of Shihezi University(Natural Science),2006,24(1):102-104. |
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| Authors: | ZHAN Yong-hua CHEN Chuang-fu GAO Jian-feng WANG Jin-fu |
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| Abstract: | This paper evaluates the quality of BAC from those aspects:the average insert size of BAC,the depth of genome coverage,the stability of BAC clones.The statistics shows: the average insert size of BAC library is 133kb and the frequency of clones without inserts is 3.2%.Thus,this library represents approximately 8-fold genome coverage.Theoretically,the probability to find any unique sequence in library screening is 99.93%.Tests on randomly selected clones demonstrated that they are very stable after serial growths. |
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| Keywords: | BAC library quality assessment ovine genome genetic stability |
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