The use of dipeptide-p-nitranilides for the study of aminopeptidase specificity |
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Authors: | M. Rybák E. Kasafirek B. Rybáková E. Simonianová |
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Affiliation: | (1) Institute of Hematology and Blood Transfusion, Research Institute for Pharmacy and Biochemistry, Post-graduate Medical Institute, Clinic of Gynaecology and Obstetrics, 128 20 Praha 2, Czechoslovakia |
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Abstract: | Summary The use of dipeptide-p-nitranilides for the study of 2 placental aminopeptidases separated on Sephadex G200 helped in establishing some regular features of their specifities. The high-molecular (320,000 daltons) one prefers Phe in position P'1 to Leu, whereas the lower-molecular aminopeptidase (145,000 daltons) prefers Leu. The high-molecular aminopeptidase splits very slowly the N-terminal Leu when Gly is in adjacent position. Leu-Gly-p-NA is therefore an inhibitor of this AP. |
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