插田泡果实花色苷萃取方法比较及微波萃取条件优化

为完善插田泡果实花色苷的萃取方法及提高花色苷得率,比较了微波辅助法、超声波辅助法、超声-微波协同萃取法3种方法对花色苷得率的影响,并选取微波功率、微波提取时间、酸化乙醇体积分数(pH值为2)3个因素进行Box-Behnken试验设计,利用响应面法对插田泡花色苷的微波萃取工艺进行优化,建立了插田泡果实花色苷得率的回归方程。结果表明,微波萃取法的花色苷得率远高于超声波辅助法及超声-微波协同萃取法。插田泡果实花色苷的优化微波萃取工艺参数为微波功率148W、微波提取时间1.10min、酸化乙醇体积分数74%(pH值为2),该条件下花色苷得率可达372.39mg/100g。

Comparison of Extraction Methods for Anthocyanins from Fruit of Rubus coreanus Maq. and Optimization of Microwave-Assisted Extraction Process

In order to improve the extraction methods and the yield of anthocyanins from fruit of Rubus coreanus Maq., the effects of microwave-assisted extraction, ultrasonic-assisted extraction and ultrasonic-microwave synergistic extraction on the yield of anthocyanins were compared. Single factor experiments were used to examine the influence of microwave power, microwave treatment time and ethanol solvent concentration (pH=2) on anthocyanin yield. The selected key parameters were optimized using response surface methodology with Box-Behnken experimental design. Results indicated that the yield of anthocyanins by microwave-assisted extraction was much higher than that of ultrasonic-assisted and ultrasonic-microwave synergistic extraction. The optimal microwave-assisted extraction parameters for anthocyanins from fruit of Rubus coreanus Maq. were microwave powder 148W, microwave treatment time 1.10min, and ethanol solvent concentration 74% (pH=2). Under the optimal extraction conditions, the anthocyanin yield from fruit of Rubus coreanus Maq. reached 372.39mg/100g.