RP-HPLC法分析罗红霉素含量

目的建立用RP-HPLC法分析罗红霉素药物含量的方法。方法以安捷伦ZORBAX Eclipse XDB—C18柱为色谱柱、乙腈（0．010moL／L）醋酸铵缓冲液（体积比70：30，pH=6．5）为流动相，选择220nm为检测波长；柱温40℃，用反相高效液相色谱法测定罗红霉素（Roxithromyein，RM）的含量。结果罗红霉素的浓度在1．150～100．8mg,／L范围内与峰面积呈良好线性关系，r=0．99977，平均回收率为99．57％，相对标准偏差为1．23％（n=5）。结论该方法操作简单、快速，可用于罗红霉素含量的测定。

Determination of roxithromycin by reversed phase high performance liquid chromatography

Objective：To establish a determination method of roxithromycin with reversed phase high performance liquid chromatography （RT-HPLC）. Methods：The RT-HPLC was performed on the Agilent ZORBAX Eclipse XDB-C18 chromatographic column, the mobile phase consisted of acetonitrile-（0.01moL/L） ammonium acetate buffer solution（70 ： 30,pH = 6.5）,the detection wavelength was 220 nm,the column temperature was 40℃. Results： There was a good linear relationship in range of 1.150-100.8 mg/L,r = 0.99977, the average recovery rate was 99.57%;the relative standard deviation was 1.23%（n=5）. Conclusion： RP-HPLC determination method of roxithromyein has the advantages of simplicity of operation and speed rapidness.