| 用于蛋白亚细胞定位研究的烟草愈伤组织培养条件优化 |
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| 引用本文： | 夏玉凤.用于蛋白亚细胞定位研究的烟草愈伤组织培养条件优化[J].河北师范大学学报(自然科学版),2006,30(3):343-345. |
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| 作者姓名： | 夏玉凤 |
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| 作者单位： | 河北师范大学,生命科学学院,河北,石家庄,050016 |
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| 基金项目： | 国家转基因植物研究和产业化专项基金 |
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| 摘 要： | 使用绿色荧光蛋白作为报告基因来研究目的蛋白的亚细胞定位得到广泛应用.使用稳定表达系统研究蛋白的亚细胞定位比较耗时,但可以先选择愈伤组织进行观察以确保构建的载体能够表达.优化了愈伤组织的培养条件,得到了质地疏松柔软的白色愈伤,不受叶绿体的荧光干扰,便于进行荧光观察.
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| 关 键 词： | 稳定表达 亚细胞定位 融合蛋白 愈伤组织 |
| 文章编号： | 1000-5854(2006)03-0343-03 |
| 收稿时间： | 2005-10-31 |
| 修稿时间： | 2005-12-12 |
Optimizing the Tobacco Callulas Culture Condition to Study Protein Subcellular Localization |
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| XIA Yu-feng.Optimizing the Tobacco Callulas Culture Condition to Study Protein Subcellular Localization[J].Journal of Hebei Normal University,2006,30(3):343-345. |
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| Authors: | XIA Yu-feng |
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| Institution: | College of Life Scienee,Hebei Normal University,Hebei Shijiazhuang 050016,China |
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| Abstract: | GFP is widely used as a report gene to study protein subcellular localization. It is time-consuming to study protein subcellular location using stable expression system. However people could select callulas as meterials to verify whether the constructed vector could be expressed in a short time. The callulas culture condition was optimized. White and soft callulas were obtained. They are fitful to be observed under fluorescence microscope, having no interference of chloroplast. |
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| Keywords: | stable expression subcellular localization fusion protein callulas |
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