三种不同诱因制作急性肺损伤动物模型的比较

摘要: 目的 对由盐酸( HC1) 、油酸( OA) 和脂多糖( LPS) 3 种诱因所致急性肺损伤( ALI) 动物模型进行比较。方法 将 24 只健康雄性 SD 大鼠随机均分为 4 组: 生理盐水( NS) 组( 尾静脉注射) 、油酸组( 尾静脉注射) 、LPS 组( 尾 静脉注射) 和 HC1 组( 气管内滴入) 。制模后观察 4 h，抽取颈总动脉血进行血气分析。然后处死，解剖取肺组织 HE 染色，观察肺组织病理形态学改变，并进行病理损伤评分。结果 HC1 组、OA 组、LPS 组氧分压( PaO2 ) 均低于 NS 组( P ＜ 0． 01) ，其中 OA 组低于 HC1 组，HC1 组低于 LPS 组，( P ＜ 0． 01) ; 肺损伤程度油酸 ＞ 盐酸 ＞ 脂多糖。结 论 ALI 的发生发展与其致病因素密切相关; 3 种诱因所致急性肺损伤动物模型存在一定差异，急性肺损伤程度不 同，油酸 ＞ 盐酸 ＞ 脂多糖。

Comparison of Three Acute Lung Injury (ALI) Rat Model Induced by Different Etiological Factors

Objective To compare three acute lung injury （ALI） rat models induced by different drugs, including hydrochloric acid （HCl）, oleinic acid （OA） and lipopolysaccharide （LPS）. Method 24 female Sprague- Dawley rats were randomly divided into 4 groups： control（NS） , HCl, OA, LPS. ALI model in HCI group was induced by hydrochloric acid via tracheal instillation, in OA group and LPS group were respectively induced by oleinic acid and lipopolysaccharide via vena caudalis, and in NS group was injected normal saline as the control. Carotid Blood samples were obtained for blood gas analysis after modeling 4 hours. Pathological changes of lung tissue were observed with HE staining and then scored by pathological injury. Result In comparing with control group, PaO2 was significantly lower in three ALI rat models （P 〈 0.01 ） , which was lower in OA group than that in HCl group （P 〈0.01 ） and lower in HCl Group than that in LPS group（P 〈0.01 ）. pathological injury score was highest in OA Group, and lowest in LPS group. Conclusion The development of ALI was closely related to the etiological factors. ALI induced with different causes has different features. The score of lung injury was OA group 〉 HCl group 〉 LPS group.