黄柏煎剂的抗炎、抗菌作用研究

摘要: 目的 观察黄柏煎剂的抗炎、抗菌作用。方法 昆明种小鼠 50 只按体质量随机分为 5 组，小鼠连续灌胃黄 柏煎剂 7 天后小鼠左耳廓内外侧涂 20 μL 二甲苯致炎，右耳不涂作为对照，计算各组耳肿胀度及抑制率; SPF 级 SD 大鼠 40 只在左、右前肢分别埋入无菌塑料环致肉芽肿，然后按体质量随机分为 5 组并按照相应剂量连续灌胃黄柏 煎剂 7 d，计算各组肉芽肿质量; 昆明种小鼠 60 只，根据性别和体质量随机分为 6 组，分别按照相应剂量连续灌胃 黄柏煎剂 7 d。第 7 天给药 1 h 后各组小鼠腹腔注射金黄色葡萄球菌菌液，6 h 再给药 1 次。观察小鼠在 72 h 内的 死亡率。结果 与对照组比较，黄柏煎剂各剂量组均能不同程度的抑制小鼠耳廓肿胀度，差异具有统计学意义( P ＜ 0. 05 或 P ＜ 0. 01) ，其中以低剂量组的作用最好; 与对照组比较，黄柏煎剂低剂量组能显著降低大鼠肉芽肿质量 ( P ＜ 0. 05) ; 高、中剂量组有一定的降低趋势，与对照组比较，差异无统计学意义( P ＞ 0. 05) ; 黄柏高、中、低剂量组 的死亡率分别是 50% 、40% 、20% 。与对照组比较，中、低剂量组可降低感染小鼠的死亡率( P ＜ 0. 05 或 P ＜ 0. 01) 。 结论 黄柏煎剂具有良好的抗炎、抗菌作用。

Effects of Cortex Phellodendri Chinensis Decoction on Anti-inflammatory and Anti-bacteria

Objective To observe the anti-inflammatory and antibacteria effect of Cortex Phellodendri Chinensis decoction（CPCD）. Method 50 Kunming mice were randomly divided into 5 groups, The mice in the study groups were administered intragastrically with CPCD, the outer ears of all mice were inflamed in left as experimental group and the right ears were not treated as a control group after treated 7 days, and then, each ear of all mice were calculated the swelling and suppression mice ; 40 SPF SD rats in the left and right forelimbs were made granulomas, and then all rats were randomly divided into five groups based on the weight, and the rats were administered intragastrically with CPCD within 7 days, then, the weight of each group granulomas were calculated; 60 Kunming mice were randomly divided into six groups according to sex and body weight, all groups mice were administered intragastrically with CPCD within 7 days according to the corresponding dose, and then, each group were administered with intraperitoneal injection of Staphylococcus aureus bacteria in the first seven days of administered after 1 h, in the first seven days of administered 6 h the mice were administered once more, mortality of mice was observed in 72 hrs. Result Experimental groups were compared with control groups, each dose group which was administered intragastrically with CPCD could inhibit ears swelling of mice in different levels, the difference was statistically significant （P 〈 O. 05 or P 〈 0.01 ） , of which the best group was low-dose group; Experimental groups were compared with the control group, low-dose groups which was administered intragastrically with CPCD could significantly reduce the weights of rat granulomas （P 〈 0.05） , and high dose group and medium dose group could not significantly reduce the weights of rat granulomas which were compared with the control group, the differences were not statistically significant （P 〉 0.05 ） ; The mice were treats with high, medium and low-dose groups, the mortality of