Oligo-1,6-glucosidase and neopullulanase enzyme subfamilies from the alpha-amylase family defined by the fifth conserved sequence region |
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Authors: | Oslancová A Janecek S |
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Institution: | (1) Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 84251 Bratislava (Slovakia), Fax + 421 2 5930 7416, e-mail: stefan.janecek@savba.sk, SLR |
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Abstract: | The α-amylase enzyme family is the largest family of glycoside hydrolases. It contains almost 30 different enzyme specificities
covering hydrolases, transferases and isomerases. Some of the enzyme specificities from the family are closely related, others
less so. This study, based on the analysis of 79 amino acid sequences, postulates two subfamilies in the framework of the
α-amylase family: the oligo-1,6-glucosidase subfamily and the neopullulanase subfamily. The specific sequence in the fifth
conserved sequence region of the family served as the basis for defining the subfamilies: QpDln for the oligo-1,6-glucosidase
subfamily and MPKln for the neopullulanase subfamily. This conserved sequence region is proposed to be the selection marker
that enables one to distinguish between the two subfamilies. The 'intermediary' sequence MPDLN can be characteristic of the
so-called intermediary group with a mixed enzyme specificity of α-amylase, cyclomaltodextrinase and neopullulanase. The evolutionary
trees clearly supported the proposed definition of the two subfamilies.
Received 12 July 2002; received after revision 28 August 2002; accepted 24 September 2002
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ID="*"Corresponding author. |
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Keywords: | , Alpha-amylase enzyme family, oligo-1,6-glucosidase, neopullulanase, conserved sequence region, evolutionary relatedness,,,,,,protein bioinformatics, |
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