首页 | 本学科首页   官方微博 | 高级检索  
     检索      


Hexosaminidase and alkaline phosphatase activities in articular chondrocytes and relationship to cell culture conditions.
Authors:E Mokonjimobe  C Hecquet  D Robic  R Bourbouze  M Adolphe
Institution:Laboratoire de Biochimie générale et métabolique, Faculté des Sciences Pharmaceutiques et Biologiques de Paris V, France.
Abstract:Hexosaminidase and alkaline phosphatase activities in rabbit articular chondrocytes have been studied under different cell culture conditions. Chondrocytes were cultured in monolayer primary culture, monolayer subcultured to the fifth passage (in vitro aging) and cultured within a collagen gel; enzymatically released cartilage cells were used as control. Under these conditions, the two enzymes behave quite differently in relationship to alteration of the chondrocyte phenotype in culture. Increased lysosomal hexosaminidase activity could be considered to be a marker of the dedifferentiated phenotype in monolayer subculture; membrane alkaline phosphatase activity could be used as a marker of non-proliferating cells.
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号