| 糖尿病家兔胰岛素受体结合性质及巯基含量的研究 |
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| 引用本文： | 葛伊莉,彭红,黄开勋. 糖尿病家兔胰岛素受体结合性质及巯基含量的研究[J]. 湖北大学学报(自然科学版), 2009, 31(1): 71-73 |
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| 作者姓名： | 葛伊莉 彭红 黄开勋 |
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| 作者单位： | 湖北大学,化学化工学院,湖北,武汉,430062;华中科技大学,化学系,湖北,武汉,430074;华中科技大学,化学系,湖北,武汉,430074 |
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| 摘 要： | 采用^125I-胰岛素标记法研究糖尿病模型家兔肝细胞膜中胰岛素受体与胰岛素的结合性质，用巯基测试法测定正常和糖尿病模型家兔肝脏胰岛素受体的巯基含量，实验证明糖尿病家兔肝细胞膜胰岛素受体不论是胰岛素的高亲和位点还是低亲和位点，胰岛素受体与胰岛素的解离都显著减弱．巯基测试结果表明糖尿病模型家兔肝脏的胰岛素受体巯基含量明显下降，提示糖尿病家兔肝脏可能存在氧化损伤．
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| 关 键 词： | 125I-胰岛素标记 胰岛索受体 巯基测试 |
The binding properties of insulin receptor and the determination of thiol group amounts in diabetic rabbits |
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| GE Yi-li,,PENG Hong,HUANG Kai-xun. The binding properties of insulin receptor and the determination of thiol group amounts in diabetic rabbits[J]. Journal of Hubei University(Natural Science Edition), 2009, 31(1): 71-73 |
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| Authors: | GE Yi-li PENG Hong HUANG Kai-xun |
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| Affiliation: | 1.School of Chemistry and Chemical Engineering;Hubei University;Wuhan 430062;China;2.Department of Chemistry;Huazhong University of Science and Technology;Wuhan 430074;China |
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| Abstract: | The binding properties of insulin receptor and insulin in liver membrane of diabetic model rabbits were investigated by 125I-insulin marking method.The thiol group amounts of insulin receptor in liver membrane of normal and diabetic model rabbits were determined by thiol group assay.The experiment showed that the dissociation of insulin receptor with insulin was obviously impaired at high and low affinity sites in the liver membrane of the diabetic rabbits.The thiol group amounts of insulin receptor in live... |
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| Keywords: | 125I-insulin marking insulin receptor thiol group determination |
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