两种洗衣粉对泥鳅血清EST同工酶表达的影响

为探讨洗衣粉对泥鳅血清同工酶表达的影响,选择两种不同的洗衣粉（代号为AJ、BJ）分别以其24 h半数致死浓度（（LC50））为参考浓度,等差设定四个剂量组（18.25 mg/L,29 mg/L,39.75 mg/L和50.5 mg/L）,采用SDS-聚丙烯酰胺凝胶（PAGE）电泳法研究了洗衣粉对泥鳅血清酯酶（EST）表达的影响.结果表明两种洗衣粉在不同浓度染毒下均使泥鳅血清酯酶（EST）同工酶的酶活性变化显著,两种洗衣粉对泥鳅血清同工酶表达具有一定的影响,且不同洗衣粉对其影响程度不同.

The Effects of Laudry Powder on the EST Isozymes in Blood of Misgurnus Anguillicaudatus

To explor the effects of laudry powder on the expression of EST isozymes. We used two kinds of laundry Powder（NO. AJand BJ）and prepared 4 concentrations of each of them according to there LC50 18.25 mg/L, 29 mg/L, 39.75 mg/L, 50. 5 mg/L）. SDS-polyarylamidegel electrophoresis test was employed to test the effects of laudry powder on the expression of EST isozymes in blood of Misgurnus anguillicaudatus. The results indicated that compared with the control group, the varieties of EST isozymes in four treatment groups appear respectively particular expression patterns in the situation of acute toxicosis. Emzyme activity of EST isozymes at four levels varies significant. Two laudry powder could all affect the expression of EST isozymes. The results also showed that the effects on the expression of EST isozymes of different type laundry powder is different.