Recombinant sTRAIL induces cell death of tumor cell lines as well as primary cancer cells |
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Authors: | Yanxin Liu Xun Zhu Zhengyu Ma Dexian Zheng |
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Institution: | (1) National Loboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 100005 Beijing, China;(2) National Laboratory of Experimental Hematology, Institue of Hematology, Chinese Academy of Medical Sciences, Peking Union Medical College, 300020 Tianjin, China |
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Abstract: | Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a new member of TNF family. It was reported that
TRAIL could induce apoptosis of tumor cells but not normal cells in tissue culture system. To further study the biological
activity and potential clinical significance, a recombinant soluble TRAIL (rsTRAIL) has been expressed stably inE. coli after transformation of pET28b vector containing the extracellular domain of TRAIL. The yield of rsTRAIL is approximately
as high as 60% of whole bacterial proteins. The rsTRAIL, purified by Ni+-agarose affinity chromatography, could remarkably trigger apoptosis at the concentrations of 0.1–1 μg/mL in all 7 tumor cell
lines testedin vitro. However, this killing activtty has not been observed in mouse fibroblast cell line (NIH3T3) as normal control. Further investigation
shows that the rsTRAIL could also kill primary tumor cells isolated freshly from patients with cardiac cancer, breast cancer
and malignant thymoma, while the normal human peripheral blood lymphocytes are not killed under the same conditions. These
results provide new evidence that rsTRAlL could induce apoptosis of tumor cells specifically and it could be a new promising
medicine for tumor therapy. |
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Keywords: | rsTRAIL apoptosis primary cancer cells |
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