基于16S rRNA和POMC基因序列的中国北方林蛙属动物系统发生关系

基于16S rRNA和POMC基因部分序列研究了中国北方林蛙属动物的系统发生关系.利用ClustalX软件对序列进行对位排列后,长度为1148bp,内群变异位点259,简约位点110.利用MP法、ML法和BI法构建系统进化树,结果显示林蛙属物种构成单系群,其中东北种群聚为一支,中国林蛙聚为一支,支持东北林蛙为有效物种.桓仁林蛙和核型为2n=26的林蛙属物种聚为一支,因此不支持核型为2n=24的林蛙形成单系群.桓仁林蛙、昆嵛林蛙、黑龙江林蛙单独聚为一支,表明三者之间的亲缘关系较近.

Phylogenetic Relationships Among Some Species of Genus rana Inferred from Partial 16S rRNA and POMC Gene Sequences

Based on partial sequences of 16S rRNA gene and POMC gene,we reconstructed phylogenetic relationships of the Chinese brown frogs(Genus rana).Sequences aligned by ClustalX showed that there were 1148 bp in length with 259 variable sites and 110 parsimony sites of the ingroup.Phylogenetic relationships were estimated by MP,ML,and BI methods.All the three phylogenetic trees strongly supported monophyly of the brown frogs.The clade of Rana chensinensis and Rana dybowskii were shown in the tree,so Rana dybowskii was supported to be anastated in this paper.Rana huanrenensis and species with 24 chromosomes were clustered together,so the monophyly of species with 24 and 22 chromosomes was not supported.Rana huanrenensis,Rana kunyuensis and Rana amurensis formed the clade in the phylogenetic trees had a very close relationships from.