| 平菇黄腐病病原菌分离与鉴定 |
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| 引用本文： | 李德舜,曹新红,苏静,王臻,张长铠.平菇黄腐病病原菌分离与鉴定[J].山东大学学报(理学版),2008,43(1):4-07. |
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| 作者姓名： | 李德舜 曹新红 苏静 王臻 张长铠 |
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| 作者单位： | 1. 山东大学微生物重点实验室,山东,济南,250100
2. 山东大学微生物重点实验室,山东,济南,250100;滨州职业学院基础部,山东,滨州,256500 |
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| 摘 要： | 本实验主要是对平菇黄腐病病原菌进行研究。从感染黄腐病的平菇幼菇子实体中分离得到2种具平板生长优势的革兰氏阴性杆状细菌(编号为G1和G2),通过对平菇子实体回接感染实验,发现细菌G1感染的平菇子实体病灶与自然感染的黄腐病状况一致,因此认为该菌就是导致平菇黄腐病的主要病原菌。经采用传统的生理生化实验与16s rDNA序列分析以及Biolog微生物自动鉴定系统分析鉴定,确定该菌为荧光假单胞菌(Pseudomonas fluorescens)。
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| 关 键 词： | 平菇黄腐病 荧光假单胞菌 回接感染 分类鉴定 |
| 文章编号： | 1671-9352（2008）01-0004-04 |
| 收稿时间： | 2007-12-17 |
| 修稿时间： | 2007年12月17 |
A pathogenic strain isolated and identified from the yellow-rotdisease of Pleurotus ostreatus |
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| LI De-shun,CAO Xin-hong,SU Jing,WANG Zhen,ZHANG Chang-kai.A pathogenic strain isolated and identified from the yellow-rotdisease of Pleurotus ostreatus[J].Journal of Shandong University,2008,43(1):4-07. |
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| Authors: | LI De-shun CAO Xin-hong SU Jing WANG Zhen ZHANG Chang-kai |
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| Institution: | 1. State Key Lab. of Microbial Technology,Shangdong University, Jinan 250100, Shandong, China;2. The Basis Department, Binzhou Vocational College, Binzhou 256500, Shandong, China |
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| Abstract: | This experiment studied the yellow-rot disease of pleurtus ostreatus. Two main strains of bacterial were isolated from cultivation plates (G1 and G2). By resumption infection experiment, it was found that the fruting bodieds of G1 are in accord with natural infection fruiting bodies. So, it is believed that G1 is the main pathogen of yellow-rot disease. Finally, this pathogen strain was identified as Pseudomonas fluorescens by traditional physiological-biochemical experiments, 16s rDNA analysis and the Biolog microbial identification system. |
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| Keywords: | pleurotus ostreatus yellow-rot disease Pseudomonas fluorescens resumption infection classification and identification |
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