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| 胞外ATP对VK2/E6E7细胞胞内pH的影响 | |
| 引用本文： | 伍忠銮,左武麟,盛雪婷,李琳,刘叔文,姜世勃,周文良. 胞外ATP对VK2/E6E7细胞胞内pH的影响[J]. 中山大学学报(自然科学版), 2008, 47(1): 123-124 |
| 作者姓名： | 伍忠銮 左武麟 盛雪婷 李琳 刘叔文 姜世勃 周文良 |
| 作者单位： | 1. 中山大学生命科学学院,广东,广州510275 2. 南方医科大学药学院,广东,广州510515 3. 南方医科大学药学院,广东,广州510515;美国纽约血液中心,纽约10021 |
| 摘 要： | 利用激光扫描共聚焦显微镜（Confocal microscope）采用pH依赖的荧光探针Snarf-4F测量细胞胞内pH(pHi)方法，发现胞外ATP能剂量依赖（10~1 000 μmol/L）地降低人阴道上皮细胞株VK2/E6E7细胞pHi。当胞外加入200 μmol/L ATP时，能快速地使VK2/E6E7细胞pHi降低，此降低的pHi在洗脱ATP后可迅速恢复。这些结果表明胞外ATP能引起VK2/E6E7细胞胞内酸化。 |
| 关 键 词： | ATP VK2/E6E7 胞内pH |
| 文章编号： | 0529-6579（2008）01-0123-02 |
| 收稿时间： | 2007-12-08 |
| 修稿时间： | 2007-12-08 |
The Effect of External ATP on Intracellular pH of VK2/E6E7 Cells |
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| WU Zhong-luan,ZUO Wu-lin,SHENG Xue-ting,LI Lin,LIU Shu-wen,JIANG Shi-bo,ZHOU Wen-liang. The Effect of External ATP on Intracellular pH of VK2/E6E7 Cells[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2008, 47(1): 123-124 | |
| Authors: | WU Zhong-luan ZUO Wu-lin SHENG Xue-ting LI Lin LIU Shu-wen JIANG Shi-bo ZHOU Wen-liang |
| Affiliation: | （1.School of Life Sciences,SUN Yat-sen University, Guangzhou 510275, China;2.School of Pharmaceutical Sciences，Southern Medical University, Guangzhou 510515, China; 3.New York Blood Center, New York 10021,USA） |
| Abstract: | Using Confocal microscope system in human vaginal epithelial cell line VK2/E6E7 cells loaded with the pH dependent fluorescent probe Snarf-4F, external ATP（10~1 000 μmol/L） was proved to dose dependently induce pHi decrease. When adding ATP (200 μmol/L) to the extracellular buffer, it induced a rapid pHi decrease and the intracellular acidification was rapidly reversed upon removal of external ATP. These results show external ATP could induce the intracellular acidification of VK2/E6E7 cells. |
| Keywords: | ATP VK2/E6E7 intracellular pH |
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