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Distribution of repetitive DNA sequences in the polytene chromosomes ofRhynchosciara americana |
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| Authors: | G M Machado-Santelli M F Bonaldo R V Santelli R A Eckhardt F J S Lara |
| Institution: | (1) Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CP 20780, São Paulo, (Brasil);(2) Present address: Fundação Centro de Pesquisa de Oncologia, Laboratorio de Oncologia Experimental da FMUSP, CP 8100, São Paulo, Brasil;(3) Present address: Department of Biology, Brooklyn College of The City University of New York, 11210 Brooklyn, New York, USA |
| Abstract: | Summary The distribution of fast, intermediate and slow renaturing fractions ofRhynchosciara americana DNA was examined in the polytene salivary gland chromosomes by in situ hybridization. Heterochromatic areas readily hybridized but hybrid formation in the euchromatin depended more on the repetitiveness of the RNA probe.Supported by funds from the Fundação de Amparo à Pesquisa do Estado de São Paulo (Projecto BIOQ-FAPESP) and from The Research Foundation of The City University of New York.R.A. Eckhardt was a visiting professor under the Multinational Scientific and Technological Development Program of the Organization of American States. |
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