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Cloning and expression of a cDNA encoding ribosomal protein S4 from Rice (Oryza sativa)
Authors:QU Lijia  LI Donghui  ZHANG Yi  LIU Meihua  GU Hongya  CHEN Zhangliang
Abstract:A cDNA clone, pS4, has been isolated from a cDNA library prepared from rice anthers of about 1.0 mm in length. DNA sequence analysis and database search show that the cDNA encodes a protein which is highly homologous to eukaryotic 80S ribosomal protein subunit 4 (S4). Northern hybridization indicates that this gene expresses in all tissues analyzed although the expression level varies and it cannot be induced by mechanical wounding in leaves. Southern blot analysis demonstrates that this rice S4 gene is from a multigene family.
Keywords:ribosomal protein S4  cDNA clone  monocotyledonous plant  tissue specificity (rice)
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