Difference in the response to PIF/activin between animal caps excised from mid- or late blastula stages ofXenopus laevis |
| |
Authors: | R. Brun A. M. Brun-Zinkernagel |
| |
Affiliation: | (1) Biology Department, Texas Christian University, 76109 Fort Worth, Texas, USA;(2) Department of Anatomy and Cell Biology, Texas College of Osteopathic Medicine, 76107 Fort Worth, Texas, USA |
| |
Abstract: | Animal caps fromXenopus embryos at Stage 7/8 cultured in salt solution, were capable of elongating, and formed various embryonic tissues including axial mesoderm, nervous tissues and pigmented retina. In contrast, animal caps from Stage 9 only developed into permanent blastulae. Following exposure to PIF/activin, however, such animal caps displayed morphogenetic movements. They formed tube-shaped embryoids that frequently had one or more tails but no head. We conclude that animal caps from Stage 9 produce more reliable results than those from Stage 7/8 because the latter are most likely contaminated with mesodermal cells. |
| |
Keywords: | Mesoderm induction Xenopus activin axes formation |
本文献已被 SpringerLink 等数据库收录! |
|