| 3,5-二硝基水杨酸法测定金钱草多糖的研究 |
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| 引用本文： | 林志銮,刘俊劭.3,5-二硝基水杨酸法测定金钱草多糖的研究[J].云南民族大学学报(自然科学版),2011,20(2). |
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| 作者姓名： | 林志銮 刘俊劭 |
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| 作者单位： | 武夷学院,环境与建筑工程系,福建省高校绿色化工技术重点实验室,福建,武夷山,354300 |
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| 摘 要： | 用溶剂法对金钱草多糖进行提取分离,研究并确定了用3,5-二硝基水杨酸(DNS)比色法测定金钱草多糖含量的方法,测定条件为:DNS最佳用量为2.0 mL,最佳显色时间为5.0 min,最优的温度为沸水加热.对金钱草多糖样品平行测定6次,相对标准偏差(RSD)为3.98%.样品加标回收率在92.52%～104.49%之间,检出限为0.022 mg/mL.
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| 关 键 词： | 金钱草 3 5-二硝基水杨酸 多糖 |
Determination of the Factors Affecting the Content of Polysaccharide in Lysimachia Christinae Hance by 3,5-Dinitrosalicylic Acid Colorimeter |
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| LIN Zhi-luan,LIU Jun-shao.Determination of the Factors Affecting the Content of Polysaccharide in Lysimachia Christinae Hance by 3,5-Dinitrosalicylic Acid Colorimeter[J].Journal of Yunnan Nationalities University:Natural Sciences Edition,2011,20(2). |
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| Authors: | LIN Zhi-luan LIU Jun-shao |
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| Institution: | LIN Zhi-luan,LIU Jun-shao(Key Laboratory for Green Chemical in Chemical Engineering and Technology Specialty of Fujian Higher Education,Department of Civil Engineering and Architecture,Wuyi university,Wuyishan 354300,China) |
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| Abstract: | |
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| Keywords: | Lysimachia christinae Hance 3 5-dinitrosalicylic acid polysaccharide |
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