应用PCR－RFLP法作脊灰炎病毒型内分析的研究

用ＰＣＲ－ＲＦＬＰ法对近年来从麻痹病人分离到的２６株脊灰炎病毒（ＰＶ）作了型内分析研究。１６株ＰＶ１的ＲＦＬＰ图式呈现多样性，毒株的图式可与Ｓａｂｉｎ１型疫苗株完全相同或不同，某些毒株亦可无电泳条带出现。７株ＰＶ２与３株ＰＶ３的ＲＦＬＰ图式则分别与疫苗株Ｓａｂｉｎ２型和３型完全相同。全面分析这些ＲＦＬＰ后，我们认为１６株ＰＶ１为野毒株，而７株ＰＶ２及３株ＰＶ３则可能是疫苗相似株。鉴于近年来广西的麻痹病例仍主要由ＰＶ１所致。故此，加强突击普服ＯＰＶ运动及作好分离毒株的型内分析已成为消灭脊灰炎的关键。

Studies on Intratypic Analysis of Poliovirus Strains by PCR-RFLP Assay

Intratypic analysis of 26 poliovirus strains isolated from paralytic poliomyelitis cases inGuangxi in the past few years had been done by PCR-RFLP assay. The results showed that RFLP pattens from 16 poliovirus type 1 strains (PV1) were various, some PV1 strains were similar or unsimilarto Sahin 1 strains'in RFLP pattern. Other PV1 strains have no bands after electrophoresis; RFLP patterns from 7 poliovirus type 2 strains (PV2) and 3 poliovirus type 3 strains (PV3) were the same asthose of Sahin 2 and Sahin 3 strains, respectively. According to the results, we think that 16 PV1 strainswould be wild poliovirus strains, 7 PV2 strains and 3 PV3 strains might be vaccine-related strains. In recent years, most paralytic poliomyelitis cases in Guangxi have been caused by PV1 wild strains. So it isvery important to strengtherl OPV vaccination arid (levelop the intratypic analysis of poliovirus strainsfor eradicating paralytic poliomyelitis.