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| 金黄色葡萄球菌isdB基因克隆、表达及其抗原性鉴定 | |
| 引用本文： | 崔莉,朱战波,朱洪伟,崔玉东,朴范泽.金黄色葡萄球菌isdB基因克隆、表达及其抗原性鉴定[J].河北科技师范学院学报,2011,25(1):16-20. |
| 作者姓名： | 崔莉 朱战波 朱洪伟 崔玉东 朴范泽 |
| 作者单位： | 1. 黑龙江八一农垦大学动物科技学院,黑龙江大庆,163319;广东温氏食品集团有限公司江苏分公司 2. 黑龙江八一农垦大学动物科技学院,黑龙江大庆,163319 3. 黑龙江八一农垦大学生命科技学院 |
| 基金项目： | 黑龙江省科技厅重大项目 |
| 摘 要： | 为了构建含牛源金黄色葡萄球菌isdB基因的原核表达载体,并确定其在大肠杆菌表达系统中的表达效果,应用PCR方法扩增出osdB基因片段与原核表达载体pQE-30,构建了重组原核表达载体pQE-30-isdB,将该重组载体转化至E.coli XL1-Blue中诱导表达蛋白.经SDS-PAGE和Westem blot鉴定,P... |
| 关 键 词： | 金黄色葡萄球菌 IsdB 原核表达 |
Prokaryotic Expression of Staphylococcus aureus isdB and Identification of Its Antigenicity |
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| CUI Li,ZHU Zhan-bo,ZHU Hong-wei,CUI Yu-dong,PIAO Fan-ze.Prokaryotic Expression of Staphylococcus aureus isdB and Identification of Its Antigenicity[J].Journal of Hebei Normal University of Science & Technology,2011,25(1):16-20. | |
| Authors: | CUI Li ZHU Zhan-bo ZHU Hong-wei CUI Yu-dong PIAO Fan-ze |
| Institution: | 1(1 College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing Heilongjiang,163319;2 Guangdong Wens Foodstuff Group Jiangsu Branch;3 College of Life Science and Technology,Heilongjiang Bayi Agricultural University;China) |
| Abstract: | To construct the prokaryotic expression vector of Staphylococcus aureus isdB gene from cow and express the gene in E.coli.,isdB gene was amplified by PCR,to expression vector pQE-30-isdB,the prokaryotic expression vector pQE-30-isdB was constructed and transformed to E.Coli XL1-Blue for expression.SDS-PAGE and Western Blot showed that a gene fragment at a length of 1 938 bp was amplified.Sequencing results showed that the isdB gene of the isolated strain shares 98.6% homology in nucleotide and amino acids sequence with that of the S.aureus strain MW2 in GenBank.The protein with a relative molecular weight of 72 ku was expressed.Western-blotting analysis indicated that the protein had antigenic activity of IsdB.This experiment successfully constructed the IsdB protein’s prokaryotic expression system. |
| Keywords: | Staphylococcus aureus IsdB prokaryotic expression |
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