| 微卫星标记在人参和西洋参鉴别中的应用 |
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| 引用本文： | 陈子易,吕旭楠,程舟,陈家宽,张文驹.微卫星标记在人参和西洋参鉴别中的应用[J].复旦学报(自然科学版),2011(2):185-191. |
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| 作者姓名： | 陈子易 吕旭楠 程舟 陈家宽 张文驹 |
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| 作者单位： | 复旦大学生命科学学院生物多样性科学研究所生物多样性与生态工程教育部重点实验室;同济大学生命科学与技术学院; |
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| 基金项目： | 上海市科学技术委员会中药现代化基金项目(08DZ1972500) |
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| 摘 要： | 从GenBank中寻找含有简单重复序列(SSR)位点的人参DNA片段序列,设计SSR引物,挑选PCR扩增条带清晰的引物,对人参和西洋参进行PCR扩增及聚丙烯酰胺凝胶电泳分析,选择能够区别2个种的引物.共有来自8个SSR位点的9对引物能扩增出可区分人参和西洋参的特异性片段,其中7对SSR引物在西洋参中能稳定地扩增出特异性...
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| 关 键 词： | 人参 西洋参 鉴别 分子标记 SSR |
Application of Microsatellite Markers to Differentiate Panax ginseng C.A.Meyer from Panax quinquefolius L. |
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| Institution: | CHEN Zi-yi1,Lü Xu-nan1,CHENG Zhou2,CHEN Jia-kuan1,ZHANG Wen-ju1(1.Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering,Institute ofBiodiversity Science,School of Life Sciences,Fudan University,Shanghai 200433,China,2.School of Life Sciences and Technology,Tongji University,Shanghai 200092,China) |
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| Abstract: | The study is to develop microsatellite markers to differentiate Panax ginseng from P.quinquefolius.Based on the sequences of P.ginseng with simple sequence repeat(SSR) in GenBank,primers were designed and tested for P.ginseng,and P.quinquefolius.Total 9 pairs of SSR primers for 8 microsatellite sites with steady and clear species-specific productions were obtained.Of these primers,7 could amplify special bands toP.quinquefolius and 2 could only amplify bands in P.ginseng.SSR-PCR provides a quick,reliable mo... |
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| Keywords: | Panax ginseng Panax quinquefolius differentiation molecular marker SSR(simple sequence repeat) |
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