| 非小细胞肺癌外周血有核细胞miRNA表达特点研究 |
| |
| 引用本文： | 王菁菁,白云,侯安继,李冲,康慧,赵国屏,王升跃. 非小细胞肺癌外周血有核细胞miRNA表达特点研究[J]. 复旦学报(自然科学版), 2011, 0(3) |
| |
| 作者姓名： | 王菁菁 白云 侯安继 李冲 康慧 赵国屏 王升跃 |
| |
| 作者单位： | 复旦大学生命科学学院微生物学与微生物工程系;国家人类基因组南方研究中心省部共建疾病与健康基因组学国家重点实验室;上海市第七人民医院; |
| |
| 基金项目： | 国家重点基础研究发展计划(973计划)项目子项目(2005CB522407); 国家青年科学基金项目(30901240); 上海市博士后科研资助计划重点项目(09R21421300); 国家博士后基金面上项目(20090450667) |
| |
| 摘 要： | miRNA在非小细胞肺癌的发生发展中发挥重要作用,为了解非小细胞肺癌患者外周血有核细胞的miRNA表达特征及其作用,采用第二代高通量测序技术对7例非小细胞肺癌患者和7例对照的外周血有核细胞miRNA表达水平进行检测,比较非小细胞肺癌患者与对照的外周血有核细胞miRNA表达特征,分析两者miRNA表达水平差异情况,共鉴定出非小细胞肺癌患者与对照的外周血有核细胞中有显著表达差异的miRNA 209种,其中肺癌样本miRNA表达上调的有138种,表达下调的有71种.研究结果显示非小细胞肺癌患者与对照样本的外周血有核细胞中miRNA表达具有显著差异,其中部分已被证实参与肿瘤发生、发展等过程.
|
| 关 键 词： | miRNA 外周血有核细胞 高通量测序 非小细胞肺癌 |
Study on miRNA Expression Profiling in the Peripheral Blood Cells With non-Small Cell Lung Cancer |
| |
| WANG Jing-jing,BAI Yun,HOU An-ji,LI Chong,KANG Hui,ZHAO Guo-ping,WANG Sheng-yue. Study on miRNA Expression Profiling in the Peripheral Blood Cells With non-Small Cell Lung Cancer[J]. Journal of Fudan University(Natural Science), 2011, 0(3) |
| |
| Authors: | WANG Jing-jing BAI Yun HOU An-ji LI Chong KANG Hui ZHAO Guo-ping WANG Sheng-yue |
| |
| Affiliation: | WANG Jing-jing1,BAI Yun2,HOU An-ji3,LI Chong1,KANG Hui2,ZHAO Guo-ping1,2,WANG Sheng-yue1,2(1.Department of Microbiology and Microbial Engineering,School of Life Science,Fudan University,Shanghai 200433,China,2.Shanghai-MOST Key Laboratory of Health and Disease Genomics,China National Human Genome Center at Shanghai,Shanghai 201203,3.Shanghai Seventh Peoples' Hospital,Shanghai 200137,China) |
| |
| Abstract: | microRNAs(miRNAs) in non-small cell lung cancer(NSCLC) have been shown to play an important role in the pathogenesis mechanism,but miRNAs expression profile in peripheral blood cells of lung cancer patients is still unknown.To detect the differentially expressed miRNAs in peripheral blood cells with NSCLC and evaluate their roles as tumor markers in lung cancer,massively parallel sequencing was be conducted to evaluate the miRNAs expression in 7 pairs of peripheral blood cells samples from NSCLC patients an... |
| |
| Keywords: | miRNA peripheral blood cells massively parallel sequencing non-small cell lung cancer |
| 本文献已被 CNKI 等数据库收录！ |
|
