酸处理对栽培条斑紫菜及其附生缘管浒苔的影响

酸处理是清除条斑紫菜(Neopyropia yezoensis)栽培过程中附生绿藻的主要方法之一，酸处理时间与pH值是影响条斑紫菜与绿藻活性的重要因素。本研究以半浮式紫菜筏架上的条斑紫菜及其附生缘管浒苔(Ulva linza)为研究对象，研究40 s浸泡时间下不同pH值的盐酸海水溶液对条斑紫菜和缘管浒苔的影响。结果显示，pH值对缘管浒苔的影响大于条斑紫菜，盐酸海水溶液浸泡40 s时间下杀灭紫菜附生缘管浒苔的最佳pH值为1.9。酸处理7 d后，条斑紫菜pH 1.9处理组的超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量、最大光化学量子效率(Fv/Fm)值以及叶绿素a(Chl a)与类胡萝卜素(Car)含量与酸处理前相比均无显著差别，而缘管浒苔的SOD、POD活性和MDA含量均显著降低，Fv/Fm值下降到0.2以下，Chl a与Car含量下降为0;在pH 1.7及以下处理组中，条斑紫菜与缘管浒苔以上生理参数与物质含量均显著低于初始值。据此并总结前人研究，推导出在pH值为0.3-1.9时，杀灭条斑紫菜附生缘管浒苔并保持紫菜活性的线性回归方程y(时间，s)=21.6...

Effects of Acid Treatment on Neopyropia Yezoensis and Its Epiphytic Ulva Linza

Acid treatment is one of the main methods to remove epiphytic green algae during the cultivation of Neopyropia yezoensis. Acid treatment time and pH value are important factors affecting the activity of N.yezoensis and green algae.In this study,N.yezoensis and its epiphytic Ulva linza on a semi-floating raft were used as research objects.The effects of hydrochloric acid seawater solution with different pH values on N.yezoensis and U.linza were studied under 40 s soaking time.The results showed that the effect of pH value on U.linza was greater than that of N.yezoensis. The optimum pH value for killing U.linza was 1.9 when immersed in hydrochloric acid seawater solution for 40 s.After 7 d of acid treatment,the activities of Superoxide Dismutase (SOD) and Peroxidase (POD),the content of Malondialdehyde (MDA),the maximum photochemical quantum yield (Fv/Fm) and the contents of Chlorophyll a (Chl a) and Carotenoid (Car) in the pH 1.9 treatment group of N.yezoensis were not significantly different from those before acid treatment.However,the activities of SOD and POD and the content of MDA in U.linza decreased significantly,the Fv/Fm value decreased to below 0.2,and the contents of Chl a and Car decreased to 0.In the pH 1.7 and below treatment group,the above physiological parameters and substance contents of N.yezoensis and U.linza were significantly lower than the initial values.Based on this and summarizing previous studies,the linear regression equation y (time,s)=21.69x(pH)-1.31 was deduced to kill U.linza and maintain the activity of N.yezoensis in the range of pH 0.3-1.9.Through the study on the physiological and biochemical response of N.yezoensis and U.linza to acid treatment,it is hoped to improve the understanding of acid treatment technology in laver cultivation,so as to make the progress of domestic laver cultivation technology.