| 用荷瘤裸鼠模型研制抗人肝癌单克隆抗体 |
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| 引用本文： | 颜江华,杨善民,林炳珍,郑耘,苏金华,陈福,陈瑞川.用荷瘤裸鼠模型研制抗人肝癌单克隆抗体[J].厦门大学学报(自然科学版),1993(3). |
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| 作者姓名： | 颜江华 杨善民 林炳珍 郑耘 苏金华 陈福 陈瑞川 |
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| 作者单位： | 厦门大学抗癌研究中心
(颜江华，杨善民，林炳珍，郑耘，苏金华，陈福)，厦门大学抗癌研究中心(陈瑞川) |
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| 摘 要： | 1985年Watanabe等报道:用去胸腺载瘤小鼠的脾细胞与小鼠骨髓瘤细胞SP 2/0融合获得抗结肠癌单克隆抗体之后,利用荷瘤裸鼠模型成功研制抗人肿瘤单抗的报道已相继出现:但所获单抗多为IgM,IgG类的单抗得率不高,本工作采用多次手术大部切除瘤块的方法延长荷瘤裸鼠的成活期,获得一株抗人肝癌IgG类单抗、其研制过程和结果报道如下。
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Preparation of Monoclonal Antibodies against Hepatocellular Carcinoma with Model of Nude Mice Bearing Tumor Xenograft |
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| Yan Jianghua Yang Shanmin Lin Binzheng Zheng YunSu Jinhua Chen Fu Chen Ruchun.Preparation of Monoclonal Antibodies against Hepatocellular Carcinoma with Model of Nude Mice Bearing Tumor Xenograft[J].Journal of Xiamen University(Natural Science),1993(3). |
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| Authors: | Yan Jianghua Yang Shanmin Lin Binzheng Zheng YunSu Jinhua Chen Fu Chen Ruchun |
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| Institution: | Cancer Research Center |
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| Abstract: | The survival time of the nude mice bearing tumor was prolonged by repeating part-cut of tumor. The spleen cells of the nude mice bearing BEL-7402 xenograft were fused with mouse myeloma cells SP2/0. One hybridomas secreting monoclonal antibody a-gainst BEL-7402 cells,MeAbJH11 ,was established. McAbJH11 is IgG3 type. Its titer of the culture supernatant and ascites was 10-2-10-3 and over 10-5,respectively. By the indirect immune-fluorescence assay,the results showed that McAbJH11 strongly reacted with BEL-7402 cells,but not with normal blood cells L342 ,MG-803,and sections of human fetal liver,spleen, lung,and so on. Our results proved that the preparation of the monoclonal antibodies with the, model of unde mice bearing tumor is an effective method. |
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| Keywords: | Monoclonal antibodies Nude mice Hepatocellular carcinoma |
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