电压门控性钾离子通道对人骨肉瘤细胞增殖的影响

研究钾离子通道在人骨肉瘤细胞的表达及通道抑制剂对人骨肉瘤细胞系增殖的影响.通过RT-PCR对三株人骨肉瘤细胞系MG63、Saos-2和SOSP-9607中相关钾离子通道,包括kv1.1, kv1.3, kv1.5, kv2.1, kv3.3/3.4, kv4.1, kv5.1, kv9.3, herg, heag 和kcnq1的基因表达进行检测;用非特异性的电压门控性钾离子通道抑制剂4-AP(4-aminopyridine,4-氨基吡啶)和氯化铯(CsCl),特异性的HERG、HEAG 、和KCa通道抑制剂E-4031、丙咪嗪(imipramine) 和TEA (tetraethylammonium,四乙铵)通过MTT法检测抑制剂对三株人骨肉瘤细胞系MG63、Saos-2和SOSP-9607细胞增殖的影响.通过RT-PCR发现kv1.3, kv1.5, kv2.1, kv3.3/3.4, kv4.1, kv9.3, herg和heag通道基因在三株骨肉瘤细胞系中均有表达,而kv5.1通道基因只在MG63细胞中表达,kcnq1通道基因只在SOSP-607和MG63细胞中表达.MTT法检测发现:E-4031, imipramine 和TEA 对三株骨肉瘤细胞的增殖没有明显的影响,而4-AP药物浓度在3 mol和5 mol时对细胞增殖影响明显.CSCL药物浓度在5 mol时对细胞增殖也有影响,但影响程度较4-AP小.提示钾离子通道在RNA水平广泛地表达于实验的三株人骨肉瘤细胞中,但只有电压门控性钾离子通道参与细胞增殖过程的调节.

Effects of Voltage-gated Potassium Channel on the Proliferation of Human Ostersarcoma

To explored the expression of potassium channels and the effects of potassium channel blockers on the proliferation in human osterosarcoma cells,the expressions of potassium channels,including kv1.1,kv1.3,kv1.5,kv2.1,kv3.3/3.4,kv4.1,kv5.1,kv9.3,herg,heag and kcnq1,were measured by RT-PCR in three human osteosarcoma cell lines of MG63,Saos-2 and SOSP-9607. Nonspecific kv channel blocker ( 4-AP and CSCl) and specific HERG,HEAG and KCa channel blocker (E-4031,imipramine and TEA respectively ) were used to exam...